Grantee Research Project Results
2013 Progress Report: Cardiometabolic, Autonomic, and Airway Toxicity of Acute Exposures to PM2.5 from Multipollutant Atmospheres in the Great Lakes Region
EPA Grant Number: R834797C002Subproject: this is subproject number 002 , established and managed by the Center Director under grant R834797
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Health Effects Institute (2005 — 2010)
Center Director: Greenbaum, Daniel S.
Title: Cardiometabolic, Autonomic, and Airway Toxicity of Acute Exposures to PM2.5 from Multipollutant Atmospheres in the Great Lakes Region
Investigators: Harkema, Jack , Fink, Greg , Wagner, James
Institution: Michigan State University
EPA Project Officer: Chung, Serena
Project Period: December 1, 2010 through November 30, 2015 (Extended to December 31, 2016)
Project Period Covered by this Report: August 1, 2012 through December 31,2013
RFA: Clean Air Research Centers (2009) RFA Text | Recipients Lists
Research Category: Human Health , Air
Objective:
Progress Summary:
Future Activities:
- Cardiotelemetry Data: Data analysis of heart rate and blood pressure responses in rats exposed to the O3/PM2.5 mixture, 5 days/wk for four weeks (Dexter inhalation exposure study) should be finished by the end of the year. In addition, data analysis of heart rate variability is being calculated for all cardiotelemetry studies and ECG waveform analyses are being planned.
- Associations of Exposure/Health Outcomes: With the help of the Biostatistics and Data Management Core, we will use data of cardiovascular responses (e.g., heart rate, mean arterial pressure, heart rate variability) and PM2.5 metrics (e.g., trace elements, gases, sources) collected over the same 30-minute timeframes to provide a high resolution correlation of specific PM2.5 components to the cardiovascular health effects. We will conduct these correlative analyses using data from both our Dearborn and Dexter studies.
- Brain Neurochemistry: With the help of collaborators and neuroscientists Drs. P.S. and Sheba MohanKumar at MSU, exposure- and diet-related responses in specific brain regions that control autonomic functions are being examined. For example, neurochemical and molecular analyses in the paraventricular nucleus of the hypothalamus are being conducted for neurotransmitters and inflammatory cytokines after inhalation exposures in both normal and high fructose-fed rats.
- Effects of Subchronic Ozone Exposure on the Development of Cardiometabolic Sydrome in C57Bl/6, KK and KKAy mice. With the help of the Biostatistics and Data Management Core, we will be completing the statistical analyses of data collected from our initial O3 studies in which we used three mouse strains to more fully understand the impact of genetics and O3 exposure on facets of CMS and diabetes.
- Initial studies on Sympathetic Neural Recordings in Rats exposed to Ozone. Dr. Fink and colleagues have been working on perfecting the surgical implantation of radiotelemetric transmitters in the renal sympathetic nerves of Sprague-Dawley rats. In late summer or early Fall 2013, we will conduct pilot studies in telemeterized rats to record changes in nerve activity during acute O3 exposure. Once successful recordings are achieved, we will move to similar studies but using acute inhalation exposures to PM2.5 and PM2.5/O3.
- Effects of Subacute PM2.5 and PM2.5/O3 Exposures on the Development of Cardiometabolic Sydrome in C57Bl/6 mice. We will test the effects of subacute PM2.5 and PM2.5/O3 inhalation exposure (4h/day, 4d/wk, for 24 consecutive weekdays) on the development of HFr-diet-induced cardiometabolic syndrome in mice. Animals
- will be fed a normal chow or an HFr diet during the inhalation exposures.
- Effects of Subacute PM2.5 and PM2.5/O3 Exposures on the Development of Cardiometabolic Sydrome in KK and KKAy mice. Based on the encouraging findings with our initial subacute O3 exposures, we will extend the inhalation toxicology studies with KK and KKAy mice by exposing these pre-diabetic strains of mice for 24 consecutive weekdays to PM2.5 and PM2.5/O3 at either our Dexter or MSU East Lansing exposure site, depending on available resources.
- Sympathetic Neural Recordings in Rodents Exposed to Ozone. We will continue to work on refining the technique of radiotelemetric microneurography in order to apply this measurement in future inhalation studies of PM2.5 and O3.
- Inhalation Toxicology Studies in Dexter, MI. In the spring and summer of 2014, we will resume inhalation toxicology studies in our rural Dexter, MI (predominantly regionally transported air pollution) to determine the cardiopulmonary, airway and metabolic effects of acute PM2.5±O3 exposures on normal- and HFr-fed rats.
Journal Articles on this Report : 7 Displayed | Download in RIS Format
Other subproject views: | All 73 publications | 28 publications in selected types | All 28 journal articles |
---|---|---|---|
Other center views: | All 148 publications | 72 publications in selected types | All 72 journal articles |
Supplemental Keywords:
Relevant Websites:
GLACIER: Great Lakes Air Center For Integrated Environmental Research Exit
Progress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R834797 Health Effects Institute (2005 — 2010) Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R834797C001 Cardiometabolic Effects of Exposure to Differing Mixtures and Concentrations of PM2.5 in Obese and Lean Adults
R834797C002 Cardiometabolic, Autonomic, and Airway Toxicity of Acute Exposures to PM2.5 from Multipollutant Atmospheres in the Great Lakes Region
R834797C003 Long Term Metabolic Consequences of Exposures to Multipollutant Atmospheres in the Great Lakes Region
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- Final Report
- 2016 Progress Report
- 2015 Progress Report
- 2014 Progress Report
- 2012 Progress Report
- 2011 Progress Report
- Original Abstract
28 journal articles for this subproject
Main Center: R834797
148 publications for this center
72 journal articles for this center