Grantee Research Project Results

2006 Progress Report: Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children

EPA Grant Number: R831861C003
Subproject: this is subproject number 003 , established and managed by the Center Director under grant R831861
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Southern California Children's Environmental Health Center
Center Director: McConnell, Rob Scot
Title: Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children
Investigators: Gilliland, Frank D.
Institution: University of Southern California
EPA Project Officer: Callan, Richard
Project Period: November 1, 2003 through October 31, 2008 (Extended to October 31, 2010)
Project Period Covered by this Report: November 1, 2005 through October 31, 2006
RFA: Centers for Children's Environmental Health and Disease Prevention Research (2003) RFA Text | Recipients Lists
Research Category: Human Health , Children's Health

Objective:

In this project, we proposed to assess the following hypotheses:
Hypothesis 1 (H1): High ambient air pollution exposure is associated with chronic airway inflammation in children as indicated by elevated eNO, a marker of airway inflammation and oxidative/nitrosative stress.
Hypothesis 2 (H2): Children's susceptibility to airway inflammation and oxidative/nitrosative stress from ambient air pollution varies by NOS1, NOS2 and NOS3, GSTM1, GSTP1, NQO1, and HO-1 genotypes.
Hypothesis 3 (H3): Children with chronic airway inflammation as indicated by elevated eNO are at increased risk for new onset asthma.

We will assess our hypotheses by accomplishing the following specific aims:
Specific Aim 1 (SA1): Collect eNO from 3000 children in the ongoing AIR study cohort.
Specific Aim 2 (SA2): Genotype the cohort of 3000 children for functional polymorphism/haplotypes in the NOS1, NOS2 and NOS3, GSTM1, GSTP1, NQO1, and HO-1.
Specific Aim 3 (SA3): To use a multilevel design to determine the relationship between levels of eNO with short- and long-term air pollution exposures and to assess the effects of genetic variation in NOS1, 2, and 3 on these relationships using data collected in SA1, SA2 and air pollution exposure estimates from the ongoing cohort study.
Specific Aim 4 (SA4): To determine the risk for new onset asthma in children with high levels of eNO using data collected in SA1 and in the ongoing prospective cohort study of incident asthma.

Progress Summary:

In this period, we have developed protocols for field collection, tested methods, and trained filed staff. We have completed fieldwork for enrolling subjects in the study and collecting eNO. We are beginning analysis of the data. Buccal cells for DNA was collected, processed and stored in the Molecular Biology Core. We developed NOS assays and are beginning to extract DNA in preparation for genotyping. We also continue to follow the cohort for asthma status and exposure variables.

Exhaled NO (eNO) has the potential for use in understanding asthma etiology, for identifying high-risk children and for use as a marker of response in intervention studies. This program of innovative research builds on the results from studies in the initial five years of the Children’s Environmental Health Center, the population resource of a large ongoing prospective cohort study of the determinants of childhood asthma incidence and an extensive cutting edge air pollution exposure assessment program to efficiently fill key research and public health needs.

Future Activities:

We plan to continue to analyzing the eNO data, complete DNA extraction and begin genotyping the cohort for the proposed genes, and continued developing exposure metrics as the exposure measurements become available. We plan to investigate the use or GIS methods to examine these data. We plan to submit a manuscript on the determinants of eNO is this large population-based sample as our first paper.

Journal Articles on this Report : 12 Displayed | Download in RIS Format

Publications Views
Other subproject views:	All 141 publications	91 publications in selected types	All 90 journal articles
Other center views:	All 202 publications	132 publications in selected types	All 131 journal articles

Publications
Type	Citation	Sub Project	Document Sources
Journal Article	Gauderman WJ, Avol E, Lurmann F, Kuenzli N, Gilliland F, Peters J, McConnell R. Childhood asthma and exposure to traffic and nitrogen dioxide. Epidemiology 2005;16(6):737-743.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (2005) R831861C001 (2006) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R827352 (Final) R827352C007 (Final) R827352C009 (Final)	Abstract from PubMed Full-text: Mothers for Clean Air Colorado-Full Text PDF Exit Abstract: Epidemiology-Abstract Exit
Journal Article	Gilliland F, Avol E, Kinney P, Jerrett M, Dvonch T, Lurmann F, Buckley T, Breysse P, Keeler G, de Villiers T, McConnell R. Air pollution exposure assessment for epidemiologic studies of pregnant women and children: lessons learned from the Centers for Children's Environmental Health and Disease Prevention Research. Environmental Health Perspectives 2005;113(10):1447-1454.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (2006) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final) R826710 (Final) R827027 (2002) R831845 (2005) R832139 (2006) R832141 (2006) R832141 (2007) R832141 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link
Journal Article	Li Y-F, Langholz B, Salam MT, Gilliland FD. Maternal and grandmaternal smoking patterns are associated with early childhood asthma. Chest 2005;127(4):1232-1241.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final)	Abstract from PubMed Full-text: Chest-Full Text HTML Exit Abstract: Chest-Abstract Exit Other: Chest-Full Text PDF Exit
Journal Article	Li Y-F, Tsao YH, Gauderman WJ, Conti DV, Avol E, Dubeau L, Gilliland FD. Intercellular adhesion molecule-1 and childhood asthma. Human Genetics 2005;117(5):476-484.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final)	Abstract from PubMed Abstract: SpringerLink-Abstract Exit
Journal Article	Li Y-F, Gauderman WJ, Avol E, Dubeau L, Gilliland FD. Associations of tumor necrosis factor G-308A with childhood asthma and wheezing. American Journal of Respiratory and Critical Care Medicine 2006;173(9):970-976.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: ATS-Full Text HTML Exit Abstract: ATS-Abstract Exit Other: ATS-Full Text PDF Exit
Journal Article	McConnell R, Berhane K, Yao L, Jerrett M, Lurmann F, Gilliland F, Kunzli N, Gauderman J, Avol E, Thomas D, Peters J. Traffic, susceptibility, and childhood asthma. Environmental Health Perspectives 2006;114(5):766-772.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (2006) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (2007) R831861C003 (Final) R826708 (Final) R827352 (Final) R827352C007 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link
Journal Article	Millstein J, Gilliland F, Berhane K, Gauderman WJ, McConnell R, Avol E, Rappaport EB, Peters JM. Effects of ambient air pollutants on asthma medication use and wheezing among fourth-grade school children from 12 Southern California communities enrolled in The Children's Health Study. Archives of Environmental Health 2004;59(10):505-514.	R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final)	Abstract from PubMed Abstract: Taylor Francis Online-Abstract Exit
Journal Article	Millstein J, Conti DV, Gilliland FD, Gauderman WJ. A testing framework for identifying susceptibility genes in the presence of epistasis. American Journal of Human Genetics 2006;78(1):15-27.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: Science Direct-Full Text HTML Exit Abstract: AJHG-Abstract Exit Other: Science Direct-Full Text PDF Exit
Journal Article	Salam MT, Li Y-F, Langholz B, Gilliland FD. Maternal fish consumption during pregnancy and risk of childhood asthma. Journal of Asthma 2005;42(6):513-518.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final)	Abstract from PubMed Abstract: Taylor and Francis-Abstract Exit
Journal Article	Salam MT, Millstein J, Li YF, Lurmann FW, Margolis HG, Gilliland FD. Birth outcomes and prenatal exposure to ozone, carbon monoxide, and particulate matter: results from the Children's Health Study. Environment Health Perspectives 2005;113(11):1638-1644.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link
Journal Article	Salam MT, Margolis HG, McConnell R, McGregor JA, Avol EL, Gilliland FD. Mode of delivery is associated with asthma and allergy occurrences in children. Annals of Epidemiology 2006;16(5):341-346.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (2006) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final)	Abstract from PubMed Abstract: Annals of Epidemiology-Abstract Exit
Journal Article	Wenten M, Berhane K, Rappaport EB, Avol E, Tsai WW, Gauderman WJ, McConnell R, Dubeau L, Gilliland FD. TNF-308 modifies the effect of second-hand smoke on respiratory illness-related school absences. American Journal of Respiratory and Critical Care Medicine 2005;172(12):1563-1568.	R831861 (2004) R831861 (2005) R831861 (2006) R831861 (Final) R831861C001 (Final) R831861C002 (Final) R831861C003 (2006) R831861C003 (Final) R826708 (2000) R826708 (2001) R826708 (2002) R826708 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: ATS Journals-Full Text PDF Exit Abstract: ATS Journals-Abstract Exit

Supplemental Keywords:

RFA, Health, Scientific Discipline, ENVIRONMENTAL MANAGEMENT, Health Risk Assessment, Children's Health, Risk Assessment, asthma, community-based intervention, airway disease, respiratory problems, Human Health Risk Assessment, childhood respiratory disease, susceptibility, children's environmental health, outreach and education

Progress and Final Reports:

Original Abstract

Main Center Abstract and Reports:

R831861 Southern California Children's Environmental Health Center

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R831861C001 Urban Air Pollution and Persistent Early Life Asthma
R831861C002 Pollution-Enhanced Allergic Inflammation and Phase II Enzymes
R831861C003 Air Pollution, Exhaled Breath Markers, and Asthma in Susceptible Children

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.