Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Identification of 'Cryptosporidium' Oocysts in River Water.
Author Ongerth, J. E. ; Stibbs, H. H. ;
CORP Author Washington Univ., Seattle.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/073;
Stock Number PB88-104161
Additional Subjects Protozoa ; Rivers ; Water pollution ; Sampling ; Particles ; Identifying ; Diseases vectors ; Humans ; Animals ; Parasites ; Chemical analysis ; Spatial distribution ; Washington(State) ; California ; Reprints ; Cryptosporidium ; Water borne diseases
Library Call Number Additional Info Location Last
NTIS  PB88-104161 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 7p
Water samples were collected from four rivers in Washington State and two rivers in California and examined for the presence of Cryptosporidium oocysts. Oocyst-sized particles were concentrated from 20-liter samples of water by membrane filtration, centrifugation, and differential sedimentation. The particle concentrate was then deposited on a 25-mm-diameter membrane filter for oocyst identification by indirect immunofluorescence assay. The identification procedure had a limit of detection of about five oocysts per liter. Cryptosporidium oocysts were found in each of 11 river water samples examined. Concentrations ranged from 2 to 112 oocysts per liter. The finding of Cryptosporidium oocysts in all samples examined from six western rivers is noteworthy in light of recent reports indicating that Cryptosporidium sp. is a significant agent of human and animal disease. This finding suggests that waterborne oocysts of this parasite are more important than was previously recognized. (Copyright (c) 1987, American Society for Microbiology.)