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Main Title Evaluation of Potential Confounding Factors in Sediment Toxicity Tests with Three Freshwater Benthic Invertebrates.
Author Ankley, G. T. ; Benoit, D. A. ; Balogh, J. C. ; Reynoldson, T. B. ; Day, K. E. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;Spectrum Research, Inc., Duluth, MN. ;National Water Research Inst., Burlington (Ontario). ;Science Applications International Corp., Hackensack, NJ.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/252;
Stock Number PB94-176138
Additional Subjects Sediments ; Invertebrates ; Toxicity ; Bias ; Amphipoda ; Physicochemical properties ; Lake sediments ; Particle size distribution ; Mortality ; Reproduction(Biology) ; Growth ; Oligochaetes ; Midges ; Bioaccumulation ; Aquatic ecosystems ; Assaying ; Lake Erie ; Lake Superior ; Lake Huron ; Lake Ontario ; Statistical analysis ; Hyalella azteca ; Chironomus tentans ; Lumbriculus variegatus
Library Call Number Additional Info Location Last
NTIS  PB94-176138 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The authors examined the effects of natural sediment physiochemical properties on the results of lab tests with the amphipod Hyalella azteca, the midge Chironomus tentans, and the oligochaete Lumbriculus variegatus. Ten-day exposures with the three species were conducted with 50 uncontaminated sediment samples from Lakes Erie, Huron, Superior, and Ontario, which differed markedly with regard to characteristics such as grain-size distribution, organic carbon content, and mineralogical composition. Tests were conducted both with and without the addition of exogenous food. Survival of Hyalella azteca, survival and growth of Chironomus tentans, and survival/reproduction and growth of Lumbriculus variegatus were significantly greater in tests in which the animals were fed vs. those in which they were not. Hence, there is significant potential for false positive results if Hyalella azteca or Chironomus tentans is not fed during sediment tests. Predictive modeling of the assay results in relationship to sediment physiochemical characteristics failed to reveal any additional factors that influenced survival of Hyalella azteca and Chironomus tentans, or reproduction and growth of Lumbriculus variegatus in tests in which the organisms were fed. However, linear modeling did suggest that growth of fed as well as unfed Chironomus tentans may have been influenced by grain-size distribution of the test sediments.