Grantee Research Project Results
Publications Details for Grant Number R833010
On-Chip PCR, Nanoparticles, and Virulence/Marker Genes for Simultaneous Detection of 20 Waterborne Pathogens and Potential Indicator Organisms
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| Reference Type | Citation | Progress Report Year | Document Sources |
|---|---|---|---|
| Book Chapter | Ahmad F, Hashsham SA. Nanosensors. In: Zhang TC, Surampalli RY, Lai KCK, Hu Z, Tyagi RD, Lo IMC, eds. Nanotechnologies for Water Environment Applications. Reston, VA: American Society of Civil Engineers, 2009, Chapter 13, pp. 412-443. |
R833010 (2009) R833010 (Final) |
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| Book Chapter | Seyrig G, Ahmad F, Stedtfeld RD, Tourlousse DM, Hashsham SA. Simple, powerful, and smart: using LAMP for low cost screening of multiple waterborne pathogens. In: Sen K, Ashbolt NJ, eds. Environmental Microbiology: Current Technology and Water Applications. Norfolk, UK: Caister Academic Press, 2011, Chapter 4, pp. 103-125. |
R833010 (Final) |
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| Journal Article | Ahmad F, Seyrig G, Tourlousse DM, Stedtfeld RD, Tiedje JM, Hashsham SA. A CCD-based fluorescence imaging system for real-time loop-mediated isothermal amplification-based rapid and sensitive detection of waterborne pathogens on microchips. Biomedical Microdevices 2011;13(5):929-937. |
EPD10016 (Final) R833010 (Final) |
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| Journal Article | Pozhitkov AE, Rule RA, Stedtfeld RD, Hashsham SA, Noble PA. Concentration dependency of nonequilibrium thermal dissociation curves in complex target samples. Journal of Microbiological Methods 2008;74(2-3):82-88. |
R833010 (2008) R833010 (Final) |
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| Journal Article | Stedtfeld RD, Baushke SW, Tourlousse DM, Miller SM, Stedtfeld TM, Gulari E, Tiedje JM, Hashsham SA. Development and experimental validation of a predictive threshold cycle equation for quantification of virulence and marker genes by high-throughput nanoliter-volume PCR on the OpenArray platform. Applied and Environmental Microbiology 2008;74(12):3831-3838. |
R831628 (2008) R833010 (2008) R833010 (Final) |
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| Presentation | Ahmad F, Stedtfeld RD, Stedtfeld TM, Thompson AL, Tourlousse DM, Tiedje JM, Hashsham SA. Towards a 1-hour genetic assay with quantitative capabilities using low-cost microchips and loop-mediated isothermal amplification. Presented at the 111th General Meeting of the American Society for Microbiology, New Orleans, LA, May 21-24, 2011. |
R833010 (Final) |
not available |
| Presentation | Hashsham SA. Parallel detection and quantification of pathogens using DNA biochips, on-chip PCR, and hand-held devices. Presented at the COST B28 Meeting, Vienna, Austria, December 10-12, 2007. |
R833010 (2008) R833010 (Final) |
not available |
| Presentation | Hashsham SA. Potential of microfluidic systems for diagnostics in plant pathology. Presented at the Canadian Phytopathological Society Annual Meeting, Prince Edward Island, Canada, June 16-18, 2008. |
R833010 (2008) R833010 (Final) |
not available |
| Presentation | Hashsham SA. Biochip analysis for microbial detection; on-chip PCR; bioindicators for pathogens. Presented at Recent Advances in Microbial Control, Arlington, VA, November 7-10, 2010. |
R833010 (Final) |
not available |
| Presentation | Hashsham SA. DNA microchips for detection of microorganisms in water and food samples. Presented at the TECAN Symposium, Salzburg, Austria, October 6-8, 2010. |
R833010 (Final) |
not available |
| Presentation | Hashsham SA, Stedtfeld RD, Ahmad F, Seyrig G, Stedtfeld TM, Tourlousse DM, Erdogan G, Tiedje JM. Merging genomics and microfluidics to develop low cost genetic analysis systems and approaches for environment and health. Presented at the BAGECO11-11th Conference on Bacterial Genetics and Ecology, BAGECO, Corfu, Greece, May 29-June 2, 2011. |
R833010 (Final) |
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| Presentation | Hashsham S. Potential of microfluidic systems for low cost diagnostics. Presented at the School of Biotechnology, Jawaharlal Nehru University, New Delhi, India, May 6, 2009. |
R833010 (2009) R833010 (Final) |
not available |
| Presentation | Pasco EV, Xagoraraki I, Hashsham SA, Bruening ML, Tarabara VV. Pathogen concentration by ultrafiltration: effects of hydraulic conditions on virus rejection and recovery. Presented at the 22nd Triennial Borchardt Conference Symposium on Advancements in Water and Wastewater, Ann Arbor, MI, February 23, 2011. |
R833010 (Final) |
not available |
| Presentation | Pasco EV, Xagoraraki I, Hashsham SA, Bruening ML, Tarabara VV. Pathogen concentration by ultrafiltration: effects of hydraulic conditions on virus rejection and recovery. Presented at the 2011 Fresh Ideas American Water Works Association (AWWA) Poster Session, AWWA/Michigan Water Environment Association (MWEA) Joint Expo, Lansing, MI, February 8-9, 2011. |
R833010 (Final) |
not available |
| Presentation | Shan W, Pasco EV, Bacchin P, Aimar P, Bruening ML, Tarabara VV. Polyelectrolyte multilayer films as backflushable nanofiltration membranes with tunable hydrophilicity and surface charge. Presented at the 11th American Water Works Association Membrane Technology Conference, Long Beach, CA, March 29, 2011. |
R833010 (Final) |
not available |
| Presentation | Stedtfeld RD, Tourlousse DM, Seyrig G, Ahmad F, Stedtfeld TM, Tiedje JM, Hashsham SA. Development and validation of a portable real time amplification-based genetic analyzer. Presented at the 111th General Meeting of American Society for Microbiology, New Orleans, LA, May 21-24, 2011. |
R833010 (Final) |
not available |
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.