| Main Title |
Evaluation of Techniques Used in the Preparation of Diesel Extract Samples for Mutagenicity Studies. |
| Author |
Williams, R. ;
Perry, E. E. ;
Lewtas, J. ;
|
| CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1986 |
| Report Number |
EPA-68-02-4031; EPA/600/J-86/360; |
| Stock Number |
PB87-193678 |
| Additional Subjects |
Exhaust gases ;
Mutagens ;
Bacteria ;
Bioassay ;
Air pollution ;
Diesel fuels ;
Preparation ;
Solvent extraction ;
Evaluation ;
Chloromethanes ;
Acetone ;
Chlorine organic compounds ;
Chlorine aliphatic compounds ;
Comparison ;
Tests ;
Salmonella ;
Reprints ;
Particulates ;
Methane/Dichloro ;
Cyclohexane
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-193678 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
Diesel exhaust particles were used to compare methods and techniques used in the preparation of particulate samples for microbial mutagenesis testing. Investigated in the study were extraction, concentration and solvent exchange methodologies as they affected recovery of mutagenic material from diesel samples using a Salmonella typhimurium plate incorporation assay. Solvent removal methods applicable for use in determining the mass concentration of extracts were also evaluated. Results indicated that particulate samples Soxhlet extracted with dichloromethane yielded higher levels of mutagenic activity than did comparative samples utilizing sonication. No difference was seen between rotary evaporation or Kuderna-Danish macro concentration of extracts to volumes greater than 50 ml. (Copyright (c) 1986 Pergamon Journals Ltd.) |
| Supplementary Notes |
Pub. in Environment International, v12 p625-633 1986. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Evaluation of Techniques Used in the Preparation of Diesel Extract Samples for Mutagenicity Studies. |
| PUB Date Free Form |
c1986 |
| Category Codes |
68D; 57K; 57B; 97K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
721924451 |
| Origin |
NTIS |
| Type |
CAT |
