||Evaluation of DNA Damage in the Oral Mucosa of Tobacco Users and Non-Users by (32)P-Adduct Assay.
Chacko, M. ;
Gupta, R. C. ;
||Baylor Univ., Houston, TX. Coll. of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
DNA adducts ;
Mouth mucosa ;
Tobacco use disorder ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Tobacco and its combustion products contain several known or potential human carcinogens and studies are not beginning to emerge for detecting DNA and protein adducts in tobacco users. A highly sensitive (32)P-adduct assay, capable of measuring a wide spectra of aromatic and/or hydrophobic DNA adducts, was used to evaluate tobacco use-related adducts in human oral mucosal cells. The chromatographic behavior of the oral mucosal DNA adducts by selective PE1-cellulose TLC and their extractability in 1-butanol suggest that they have an aromatic and/or hydrophobic moiety and two of the major adducts have been tentatively assigned as aromatic amine derivatives.
||Pub. in Carcinogenesis, v9 n12 p2309-2314 1988. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Evaluation of DNA Damage in the Oral Mucosa of Tobacco Users and Non-Users by (32)P-Adduct Assay.
||57E; 57D; 57F; 57Y
||PC A02/MF A01