| Main Title |
Formation of Cigarette Smoke-Induced DNA Adducts in the Rat Lung and Nasal Mucosa. |
| Author |
Gupta, R. C. ;
Sopori, M. L. ;
Gairola., C. G. ;
|
| CORP Author |
Baylor Coll. of Medicine, Houston, TX. Dept. of Pharmacology. ;Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM. ;Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA/600/J-89/193; |
| Stock Number |
PB90-140799 |
| Additional Subjects |
Deoxyribonucleic acids ;
Smoking ;
Lung ;
Liver ;
Rats ;
Respiration ;
Exposure ;
Blood ;
Reprints ;
DNA adducts ;
Nasal mucosa ;
DNA damage ;
Dose-response relationships ;
Carboxyhemoglobin
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-140799 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
The formation of DNA adducts in the nasal, lung, and liver tissues of rats exposed daily to fresh smoke from a University of Kentucky reference cigarette (2R1) for up to 40 weeks was examined. The amount of smoke total particulate matter (TPM) inhaled and the blood carboxyhemoglobin (COHb) values averaged 5-5.5 mg smoke TPM/day/rat and 5.5%, respectively. The pulmonary AHH activity measured at the termination of each experiment showed an average increase of about two- to threefold in smoke-exposed groups. These observations suggested that animals effectively inhaled both gaseous and particulate phase constituents of cigarette smoke. DNAs from nasal, lung, and liver tissue were extracted and analyzed by an improved 32P-postlabeling procedure. The data demonstrates the DNA-damaging potential of long term fresh cigarette smoke exposure and suggest the ability of the tissue to partially recover from such damage following cessation of the exposure. |
| Supplementary Notes |
Pub. in Cancer Research 49, p1916-1920, 15 Apr 89. Prepared in cooperation with Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM., and Kentucky Univ., Lexington. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Formation of Cigarette Smoke-Induced DNA Adducts in the Rat Lung and Nasal Mucosa. |
| Category Codes |
57Y; 68G |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
005320351 |
| Origin |
NTIS |
| Type |
CAT |
