||Mutagenicity Evaluation of Chlorendic Anhydride in the Unscheduled DNA Synthesis in Human WI-38 Cells Assay (Final Report) with Attachments and Cover Letter Dated 06/15/78.
||Litton Bionetics, Inc., Kensington, MD. ;Velsicol Chemical Corp., Chicago, IL.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
||28 Jun 1978
Deoxyribonucleic acid ;
Cell cultures ;
Molecular biology ;
Chlorendic anhydride ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The test material was evaluated for its ability to induce unscheduled DNA synthesis in human WI-38 cells blocked in G1 phase. The results area presented in Tables 1 and 2. The test substance was prepared as a stock solution in DMSO. The stock solution was diluted in culture medium to derive the final test concentrations. The range of concentrations employed in this assay were from 0.01 mg/ml to 0.5 mg/ml. The only evidence of toxicity was observed at the highest dose level of 0.5 mg/ml. The evidence of toxicity was derived from the reduction in counts (CPM) below the control. The results of the test are shown in Tables 1 and 2. They are presented in tabular and graphic forms. The data from this assay showed significant increases in UDS both with and without a microsome S9 preparation. The activation test conditions provided the greatest increases over control.