| Main Title |
Reliable, Efficient, Microinjection Apparatus and Methodology for the In vivo Exposure of Rainbow Trout and Salmon Embryos to Chemical Carcinogens. |
| Author |
Black, J. J. ;
Maccubbin, A. E. ;
Schiffert., M. ;
|
| CORP Author |
Roswell Park Memorial Inst., Buffalo, NY. Dept. of Experimental Biology.;Environmental Research Lab.-Duluth, MN. |
| Publisher |
c1985 |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/552; |
| Stock Number |
PB90-146267 |
| Additional Subjects |
Carcinogens ;
Embryos ;
Fishes ;
Equipment ;
Injections(Medicine) ;
Trout ;
Salmon ;
Mortality ;
Optimization ;
Survival ;
Neoplasms ;
Humans ;
Indicator species ;
Exposure ;
Methyl sulfoxide ;
Tests ;
Reliability ;
Salmo gairdneri
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-146267 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
A modular apparatus and technique for the injection of salmonid fish embryos with chemical carcinogens are described. A key feature of the methodology is the relative ease of routine through-the-eggshell injection, into the yolk sac of living salmonid fish embryos, inside the 'eyed-stage' egg. The procedure is sufficiently rapid that 2 persons working as a team can give injections to 200 embryos per hour. The injection per se induces low mortality, i.e., optimal net survival rates (controls given an injection of dimethyl sulfoxide vs. those not given an injection) in the range of 70-90%. |
