Record Display for the EPA National Library Catalog


Main Title Standard test fish development : part I, fathead minnows (Pimephales promelas) and goldfish (Carassius auratus) as standard fish in bioassays and their reaction to potential reference toxicants /
Author Adelman, Ira R. ; Smith, Jr., Lloyd L.
Other Authors
Author Title of a Work
Smith, Lloyd L.
Smith, Lloyd LeRoy
CORP Author Environmental Research Laboratory (Duluth, Minn.); University of Minnesota. Dept. of Entomology, Fisheries, and Wildlife.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory ; available through the National Technical Information Service,
Year Published 1976
Report Number EPA/600/3-76/061a; EPA-R-800940
Stock Number PB-258 099
OCLC Number 05290539
Subjects Water--Pollution--Measurement ; Water quality bioassay ; Minnows ; Goldfish
Additional Subjects Water--Pollution--Measurement ; Water quality bioassay ; Minnows ; Goldfish ; Toxicity ; Fishes ; Sodium chloride ; Chromium ; Bioassay ; Lethal dosage ; Age ; Standards ; Tests ; Chemical compounds ; Fathead minnows ; Toxic substances ; Phenols/chloro ; Guthion
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJED  EPA 600/3-76-061a OCSPP Chemical Library/Washington,DC 01/01/1988
NTIS  PB-258 099 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation x, 77 p. : ill. ; 28 cm.
Fathead minnows and goldfish were compared for their suitability as a standard bioassay fish. Acute bioassays of four potential reference toxicants, sodium chloride, pentachlorophenol, hexavalent chromium, and Guthion, were conducted with both species, and results were reported as toxicity curves as well as LC50's at various times. Both species showed the same variability of bioassay results. Since goldfish could not complete a life cycle in 1 year under laboratory conditions, fathead minnows were recommended as a standard species on the basis of their smaller size and their utility in complete life cycle tests. Bioassays of pentachlorophenol were conducted with both species to determine the effect of testing different sized fish of the same age or testing different aged fish. Size selection of fish within the ranges tested is unnecessary since differences in LC50's were small. Since age of fathead minnows did not affect the LC50's after 24 hours, use of younger fish would allow smaller bioassay chambers or more fish per chamber. On the basis of seven criteria, sodium chloride was superior for use as a reference toxicant with pentachlorophenol a close second choice.
Submitted by Department of Entomology, Fisheries, and Wildlife, University of Minnesota in fulfillment of project 18050 H0H and grant no. R800940. Includes bibliographies.