Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Application of the 'Tradescantia micronucleus' Assay for the Genetic Evaluation of Chemical Mixtures in Soil and Aqueous Media.
Author Gill, B. S. ; Sandhu, S. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/439;
Stock Number PB93-141380
Additional Subjects Micronucleus tests ; Mutagens ; Soil contamination ; Water pollution effects(Plants) ; Arsenic oxides ; Dieldrin ; Reprints ; Tradescantia ; Lead tetraacetate
Library Call Number Additional Info Location Last
NTIS  PB93-141380 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/12/1993
Collation 7p
Genotoxic evaluation of nine binary and one tertiary mixtures of arsenic trioxide, dieldrin, and lead tetraacetate were performed using the Tradescantia micronucleus (Trad-MN) assay. The chemical mixtures were either (1) mixed into soil, and chemical exposure to the target cells was through the roots of intact plants grown in the soil or (2) through stem cuttings in which the inflorescences were treated with an aqueous solution of the test chemicals. Clastogenic responses were obtained for six out of the ten chemical mixtures, irrespective of the treatment conditions although relatively higher frequencies of MN were observed in plants exposed to the test chemicals in soil. The clastogenicity of the chemical mixtures was modified by the ratio of the individual chemical in a particular mixture. The results of this study suggest that in addition to its utility for monitoring the presence of clastogens in ambient air and water, the Trad-MN assay also be used as a reliable test system for detecting the levels of clastogens in soil.