| Main Title |
Mutagenicity Evaluation of Chlorendic Anhydride in the Unscheduled DNA Synthesis in Human WI-38 Cells Assay (Final Report) with Attachments and Cover Letter Dated 06/15/78. |
| CORP Author |
Litton Bionetics, Inc., Kensington, MD. ;Velsicol Chemical Corp., Chicago, IL.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Publisher |
28 Jun 1978 |
| Year Published |
1978 |
| Report Number |
8EHQ-0678-0206; |
| Stock Number |
OTS-0200584 |
| Additional Subjects |
Anhydrides ;
Deoxyribonucleic acid ;
Cell cultures ;
Humans ;
Synthesis ;
Toxicity ;
Assaying ;
Dosage ;
Concentration(Compositions) ;
Evaluation ;
Molecular biology ;
Mutatgenesis ;
Powders ;
Chlorendic anhydride ;
White powder
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS-0200584 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
72p |
| Abstract |
The test material was evaluated for its ability to induce unscheduled DNA synthesis in human WI-38 cells blocked in G1 phase. The results area presented in Tables 1 and 2. The test substance was prepared as a stock solution in DMSO. The stock solution was diluted in culture medium to derive the final test concentrations. The range of concentrations employed in this assay were from 0.01 mg/ml to 0.5 mg/ml. The only evidence of toxicity was observed at the highest dose level of 0.5 mg/ml. The evidence of toxicity was derived from the reduction in counts (CPM) below the control. The results of the test are shown in Tables 1 and 2. They are presented in tabular and graphic forms. The data from this assay showed significant increases in UDS both with and without a microsome S9 preparation. The activation test conditions provided the greatest increases over control. |
