Record Display for the EPA National Library Catalog


Main Title Mutagenicity Evaluation of Chlorendic Anhydride in the Unscheduled DNA Synthesis in Human WI-38 Cells Assay (Final Report) with Attachments and Cover Letter Dated 06/15/78.
CORP Author Litton Bionetics, Inc., Kensington, MD. ;Velsicol Chemical Corp., Chicago, IL.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Publisher 28 Jun 1978
Year Published 1978
Report Number 8EHQ-0678-0206;
Stock Number OTS-0200584
Additional Subjects Anhydrides ; Deoxyribonucleic acid ; Cell cultures ; Humans ; Synthesis ; Toxicity ; Assaying ; Dosage ; Concentration(Compositions) ; Evaluation ; Molecular biology ; Mutatgenesis ; Powders ; Chlorendic anhydride ; White powder
Library Call Number Additional Info Location Last
NTIS  OTS-0200584 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 72p
The test material was evaluated for its ability to induce unscheduled DNA synthesis in human WI-38 cells blocked in G1 phase. The results area presented in Tables 1 and 2. The test substance was prepared as a stock solution in DMSO. The stock solution was diluted in culture medium to derive the final test concentrations. The range of concentrations employed in this assay were from 0.01 mg/ml to 0.5 mg/ml. The only evidence of toxicity was observed at the highest dose level of 0.5 mg/ml. The evidence of toxicity was derived from the reduction in counts (CPM) below the control. The results of the test are shown in Tables 1 and 2. They are presented in tabular and graphic forms. The data from this assay showed significant increases in UDS both with and without a microsome S9 preparation. The activation test conditions provided the greatest increases over control.