Record Display for the EPA National Library Catalog


Main Title Evaluation of Techniques Used in the Preparation of Diesel Extract Samples for Mutagenicity Studies.
Author Williams, R. ; Perry, E. E. ; Lewtas, J. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-68-02-4031; EPA/600/J-86/360;
Stock Number PB87-193678
Additional Subjects Exhaust gases ; Mutagens ; Bacteria ; Bioassay ; Air pollution ; Diesel fuels ; Preparation ; Solvent extraction ; Evaluation ; Chloromethanes ; Acetone ; Chlorine organic compounds ; Chlorine aliphatic compounds ; Comparison ; Tests ; Salmonella ; Reprints ; Particulates ; Methane/Dichloro ; Cyclohexane
Library Call Number Additional Info Location Last
NTIS  PB87-193678 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Diesel exhaust particles were used to compare methods and techniques used in the preparation of particulate samples for microbial mutagenesis testing. Investigated in the study were extraction, concentration and solvent exchange methodologies as they affected recovery of mutagenic material from diesel samples using a Salmonella typhimurium plate incorporation assay. Solvent removal methods applicable for use in determining the mass concentration of extracts were also evaluated. Results indicated that particulate samples Soxhlet extracted with dichloromethane yielded higher levels of mutagenic activity than did comparative samples utilizing sonication. No difference was seen between rotary evaporation or Kuderna-Danish macro concentration of extracts to volumes greater than 50 ml. (Copyright (c) 1986 Pergamon Journals Ltd.)