Record Display for the EPA National Library Catalog


Main Title Review of Methods That Are Used to Determine 'Giardia' Cyst Viability.
Author Schaefer, F. W. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1987
Report Number EPA/600/D-87/096;
Stock Number PB87-174843
Additional Subjects Giardia ; Cysts ; Viability ; Reviews ; Methodology ; Microscopy ; Fluorescent dyes ; Infectious diseases ; Parasites ; Transmission ; Disinfection ; Excystation
Library Call Number Additional Info Location Last
NTIS  PB87-174843 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 27p
Over the past 55 years a number of methods have been described for excysting Giardia cysts as a means of determining viability. The excystation methods for G. muris cysts are reliable and reproducible. However, methods published to date for the excystation of G. lamblia cysts, the human pathogen, have not yielded reliable, reproducible results. For both Giardia cyst types the stimulatory factors promoting excystation are low pH, carbon dioxide, a temperature around 37 C, and a final neutralizing step at pH 7.0. Recently, other methods have been developed for determining Giardia cyst viability. These include several fluorescent vital dyes, differential interference contrast microscopy, and Mongolian gerbil infectivity tests.