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Main Title Effect of Solvent and Extraction Methods on the Bacterial Mutagenicity of Sidestream Cigarette Smoke.
Author Morin, R. S. ; Tulis, J. J. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Bioassay Branch. ;North Carolina Univ. at Chapel Hill. School of Public Health.
Year Published 1987
Report Number EPA/600/J-87/256;
Stock Number PB88-182381
Additional Subjects Mutagens ; Bacteria ; Solvent extraction ; Smoke ; Air pollution ; Toxicity ; Cigarettes ; Genetics ; Particles ; Laboratories ; Metabolism ; Salmonella ; Activation ; Agitation ; Ultrasonic frequencies ; Acetone ; Carbinols ; Chloromethanes ; Reprints ; Controlled burning ; Ames test ; Salmonella typhimurium
Library Call Number Additional Info Location Last
NTIS  PB88-182381 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
The mutagenic activity of sidestream cigarette smoke particles was estimated by testing sidestream cigarette smoke particles which had been collected under controlled burning conditions in the laboratory. Two different extraction methods (Soxhlet and ultrasonic agitation) and 3 different solvents (dichloromethane, methanol, and acetone) were compared for their efficiencies in the extraction of compounds from sidestream cigarette smoke particles which are mutagenic in the Ames test. The mutagenic activity of the sidestream smoke particles was estimated to be 15,000-20,000 revertants per cigarette in TA98 with metabolic activation and 12,000-17,000 revertants per cigarette in TA100 without metabolic activation. (Copyright (c) 1987 Elsevier Science Publishers B.V. (Biomedical Division).)