Record Display for the EPA National Library Catalog


Main Title Mammalian Cell Culture Assay to Quantitate Chemically Induced Aneuploidy: Use of a Monochromosomal Human/Mouse Cell Hybrid.
Author Athwal, R. S. ; Sandhu, S. S. ;
CORP Author New Jersey Medical School, Newark. Dept. of Microbiology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/D-85/019;
Stock Number PB85-161370
Additional Subjects Assays ; Cell cultures ; Mammals ; Humans ; Mice ; Cell lines ; Aneuploidy ; Cytogenetics
Library Call Number Additional Info Location Last
NTIS  PB85-161370 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 24p
A short-term assay utilizing a human/mouse monochromosomal hybrid cell line R3-5, to detect chemically induced aneuploidy in mammalian cells is described. A single human chromosome transferred into mouse cells was used as a cytogenetic marker to quantitate abnormal chromosome segregation following chemical treatment. The human chromosome present in the mouse cells can be readily identified by differential staining procedures. The frequency of cells containing 0 or 2 human chromosomes in the progeny of chemically-treated monochromosomal hybrid cells provided a direct measure of aneuploidy. We tested the sensitivity of the proposed system with three model chemicals (Colcemid, cyclophosphamide and benomyl) known to induce numerical or structural changes in chromosomes. The frequency of an abnormal segregation of the human chromosome was found to be dose dependent and consistently higher than controls. This system has the capability to detect gain as well as loss of a chromosome resulting from nondisjunction or other mechanisms leading to aneuploidy.