Grantee Research Project Results
2012 Progress Report: Arsenic and Maternal and Infant Immune Function
EPA Grant Number: R834599Center: Children's Environmental Health and Disease Prevention Center - Dartmouth College
Center Director: Karagas, Margaret Rita
Title: Arsenic and Maternal and Infant Immune Function
Investigators: Karagas, Margaret Rita , Purvis, Lisa A. , Korrick, Susan A. , Moeschler, John B. , Enelow, Richard I. , Robbins, David J , Folt, Carol L. , Cottingham, Kathryn L. , Onega, Tracy L. , Gui, Jiang , Rees, Judy , Madan, Juliette , Punshon, Tracy , Shi, Xun
Current Investigators: Karagas, Margaret Rita , Purvis, Lisa A. , Korrick, Susan A. , Moeschler, John B. , Enelow, Richard I. , Robbins, David J , Folt, Carol L. , Cottingham, Kathryn L. , Onega, Tracy L. , Gui, Jiang , Rees, Judy , Madan, Juliette , Miller, Stephanie , Punshon, Tracy , Shi, Xun
Institution: University of Miami , Dartmouth Medical School , Dartmouth College
Current Institution: Dartmouth College , Dartmouth Medical School , University of Miami
EPA Project Officer: Callan, Richard
Project Period: February 15, 2010 through February 14, 2013 (Extended to February 14, 2014)
Project Period Covered by this Report: February 15, 2012 through February 14,2013
Project Amount: $1,079,663
RFA: Children's Environmental Health and Disease Prevention Research Centers: Formative Centers (with NIEHS) (2009) RFA Text | Recipients Lists
Research Category: Children's Health , Human Health
Objective:
- To begin evaluating how increased infant consumption of solid foods affects arsenic exposure for infants aged 4-12 months
- To test the feasibility of measuring urinary metabolites of arsenic in infants at 4 months of age, and determine the relationships among urinary arsenic, arsenic ingestion estimated from food and water, and toenail arsenic levels.
Progress Summary:
- For the data of arsenic concentration in groundwater, we acquired three most updated (unpublished) new GIS data layers from USGS NH-VT Water Science Center, representing the probabilities of arsenic concentration > 1, 5, and 10 ug/L, respectively, at each location (represented by 30 m pixel) in New Hampshire.
- For the public water supply coverage in NH, we acquired public water supply pipeline data from the NH Department of Environmental Service.
- We further compiled the birth defect data of NH for geospatial analysis. Besides the filtering processes reported last time (unique infant, plurality, mothers age, etc), we did literature research and had extensive discussions on identifying non-environmental birth defect types. Therefore, we excluded fetal alcohol syndrome and chromosomal defects (i.e., Down syndrome, Trisomy 13, and Trisomy 18, accounting for about 8% of all the records) because these conditions result from known causes.
- We have compiled data for studies of low birth weight (LBW), high birth weight (HBW), and small for gestational age (SGA) in NH.
- We further developed and improved the sophisticated geocomputational approach to disease mapping and have named it the RCMC-UCMC approach (restricted-and-controlled-Monte-Carlo and unrestricted-and-controlled-Monte-Carlo). We applied it to NH birth defect mapping and created high- resolution risk maps of birth defects in NH, along with quantified evaluation of spatial uncertainty of the results. A paper of this work has been submitted to the American Journal of Epidemiology. We have established collaboration with the National Center for Supercomputing Applications (NCSA), located at the University of Illinois at Urbana-Champaign, to migrate the software we developed for this method to the Centers supercomputing environment, so as to enhance its computational capability and public accessibility.
- We conducted a fairly exhaustive exploration for detecting spatial association between groundwater arsenic and birth defects in NH.
- On the arsenic side, we tested the well data collected by the Dartmouth researchers (led by Margaret Karagas, the PI of this Center), and four different USGS datasets (raster layers of probabilities for different arsenic concentration levels).
- For the public water coverage, using the best available public water supply pipeline data from the NH DES, we tested a number of different buffer distances around the pipelines to define the public water supply coverage.
- For confounding factors, besides mother's age, we tested land use (different degrees of urbanization), household medium income, and total population.
- For the geographic scale, we conducted analyses at both the town level and very detailed pixel level (30 m).
- For NH, we are conducting town-level analysis on spatial association between low birth weight (LBW) and groundwater arsenic, and a paper is under preparation.
- The analyses for high birth weight (HBW) and small for gestational age (SGA) in NH are ongoing.
- We have also acquired data of state Maine. The compilation of Maine data is largely done, and the analysis has started.
Future Activities:
Project 2 (R834599C002)
We will continue to collect data on infant consumption of breast milk and formula via telephone and collect food diaries and urine samples at 6 weeks as additional infants are enrolled in this study. In addition, we will begin to collect food diaries, urine samples, and toenail clippings at 12 months.Project 4: (R834599C004)
- Publish our manuscript on arsenic-related genes in the placenta in relation to birth outcomes.
- Submit a manuscript on the modulation of key developmental signaling target genes by arsenic.
- Continue to develop the chick embryo explant system, as a relevant developmental model system.
Journal Articles: 29 Displayed | Download in RIS Format
Other center views: | All 76 publications | 29 publications in selected types | All 29 journal articles |
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Carey AM, Norton GJ, Deacon C, Scheckel KG, Lombi E, Punshon T, Guerinot ML, Lanzirotti A, Newville M, Choi Y, Price AH, Meharg AA. Phloem transport of arsenic species from flag leaf to grain during grain filling. New Phytologist 2011;192(1):87-98. |
R834599 (2012) R834599C002 (2012) |
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Carey A-M, Lombi E, Donner E, de Jonge MD, Punshon T, Jackson BP, Guerinot ML, Price AH, Meharg AA. A review of recent developments in the speciation and location of arsenic and selenium in rice grain. Analytical and Bioanalytical Chemistry 2012;402(10):3275-3286. |
R834599 (2012) R834599 (Final) R834599C002 (2012) R834599C002 (Final) |
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Cottingham KL, Karimi R, Gruber JF, Zens MS, Sayarath V, Folt CL, Punshon T, Morris JS, Karagas MR. Diet and toenail arsenic concentrations in a New Hampshire population with arsenic-containing water. Nutrition Journal 2013;12:149. |
R834599 (2011) R834599 (Final) R834599C001 (Final) R834599C002 (Final) R835442 (2014) R835442 (2015) R835442 (2016) |
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Davis MA, Mackenzie TA, Cottingham KL, Gilbert-Diamond D, Punshon T, Karagas MR. Rice consumption and urinary arsenic concentrations in U.S. children. Environmental Health Perspectives 2012;120(10):1418-1424. |
R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) R834599C002 (2012) R834599C002 (Final) |
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Farzan SF, Karagas MR, Chen Y. In utero and early life arsenic exposure in relation to long-term health and disease. Toxicology and Applied Pharmacology 2013;272(2):384-390. |
R834599 (Final) R834599C001 (Final) |
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Farzan SF, Korrick S, Li Z, Enelow R, Gandolfi AJ, Madan J, Nadeau K, Karagas MR. In utero arsenic exposure and infant infection in a United States cohort: a prospective study. Environmental Research 2013;126:24-30. |
R834599 (Final) R834599C001 (Final) |
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Fei DL, Sanchez-Mejias A, Wang Z, Flaveny C, Long J, Singh S, Rodriguez-Blanco J, Tokhunts R, Giambelli C, Briegel KJ, Schulz WA, Gandolfi AJ, Karagas M, Zimmers TA, Jorda M, Bejarano P, Capobianco AJ, Robbins DJ. Hedgehog signaling regulates bladder cancer growth and tumorigenicity. Cancer Research 2012;72(17):4449-4458. |
R834599 (2012) R834599 (Final) R834599C004 (2012) R834599C004 (Final) |
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Fei DL, Koestler DC, Li Z, Giambelli C, Sanchez-Mejias A, Gosse JA, Marsit CJ, Karagas MR, Robbins DJ. Association between In Utero arsenic exposure, placental gene expression, and infant birth weight: a US birth cohort study. Environmental Health 2013;12:58 (8 pp.). |
R834599 (Final) R834599C001 (Final) R834599C004 (Final) R835442 (2014) R835442 (2015) R835442 (2016) |
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Gilbert-Diamond D, Cottingham KL, Gruber JF, Punshon T, Sayarath V, Gandolfi AJ, Baker ER, Jackson BP, Folt CL, Karagas MR. Rice consumption contributes to arsenic exposure in US women. Proceedings of the National Academy of Sciences of the United States of America 2011;108(51):20656-20660. |
R834599 (2011) R834599 (2012) R834599 (Final) R834599C001 (Final) R834599C002 (2012) R834599C002 (Final) |
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Gruber JF, Karagas MR, Gilbert-Diamond D, Bagley PJ, Zens MS, Sayarath V, Punshon T, Morris JS, Cottingham KL. Associations between toenail arsenic concentration and dietary factors in a New Hampshire population. Nutrition Journal 2012;11:45 (10 pp.). |
R834599 (2011) R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) R834599C002 (2012) R834599C002 (Final) |
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Jackson BP, Taylor VF, Punshon T, Cottingham KL. Arsenic concentration and speciation in infant formulas and first foods. Pure and Applied Chemistry 2012;84(2):215-223. |
R834599 (2012) R834599 (Final) R834599C002 (2012) R834599C002 (Final) |
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Jackson BP, Taylor VF, Karagas MR, Punshon T, Cottingham KL. Arsenic, organic foods, and brown rice syrup. Environmental Health Perspectives 2012;120(5):623-626. |
R834599 (2011) R834599 (2012) R834599 (Final) R834599C001 (Final) R834599C002 (2012) R834599C002 (Final) |
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Karagas MR. Arsenic-related mortality in Bangladesh. The Lancet 2010;376(9737):213-214. |
R834599 (Final) R834599C001 (Final) |
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Karagas MR, Wasson JH. A World Wide Web-based survey of non-medical tattooing in the United States. Journal of the American Academy of Dermatology 2012;66(1):e13-e14. |
R834599 (2011) R834599 (Final) R834599C001 (Final) |
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Karagas MR, Choi AL, Oken E, Horvat M, Schoney R, Kamai E, Cowell W, Grandjean P, Korrick S. Evidence on the human health effects of low-level methylmercury exposure. Environmental Health Perspectives 2012;120(6):799-806. |
R834599 (2011) R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) |
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Karagas MR, Andrew AS, Nelson HH, Li Z, Punshon T, Schned A, Marsit CJ, Morris JS, Moore JH, Tyler AL, Gilbert-Diamond D, Guerinot ML, Kelsey KT. SLC39A2 and FSIP1 polymorphisms as potential modifiers of arsenic-related bladder cancer. Human Genetics 2012;131(3):453-461. |
R834599 (2011) R834599 (Final) R834599C001 (Final) R834599C002 (Final) |
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Koestler DC, Christensen B, Karagas MR, Marsit CJ, Langevin SM, Kelsey KT, Wiencke JK, Houseman EA. Blood-based profiles of DNA methylation predict the underlying distribution of cell types: a validation analysis. Epigenetics 2013;8(8):816-826. |
R834599 (Final) R834599C001 (Final) |
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Koestler DC, Avissar-Whiting M, Houseman EA, Karagas MR, Marsit CJ. Differential DNA methylation in umbilical cord blood of infants exposed to low levels of arsenic in utero. Environmental Health Perspectives 2013;121(8):971-977. |
R834599 (2011) R834599 (Final) R834599C001 (Final) R835442 (2014) R835442 (2015) R835442 (2016) |
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Lesseur C, Gilbert-Diamond D, Andrew AS, Ekstrom RM, Li Z, Kelsey KT, Marsit CJ, Karagas MR. A case-control study of polymorphisms in xenobiotic and arsenic metabolism genes and arsenic-related bladder cancer in New Hampshire. Toxicology Letters 2012;210(1):100-106. |
R834599 (2011) R834599 (Final) R834599C001 (Final) |
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Madan JC, Farzan SF, Hibberd PL, Karagas MR. Normal neonatal microbiome variation in relation to environmental factors, infection and allergy. Current Opinion in Pediatrics 2012;24(6):753-759. |
R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) |
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Madan JC, Koestler DC, Stanton BA, Davidson L, Moulton LA, Housman ML, Moore JH, Guill MF, Morrison HG, Sogin ML, Hampton TH, Karagas MR, Palumbo PE, Foster JA, Hibberd PL, O'Toole GA. Serial analysis of the gut and respiratory microbiome in cystic fibrosis in infancy: interaction between intestinal and respiratory tracts and impact of nutritional exposures. mBio 2012;3(4):e00251-12 (10 pp.). |
R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) |
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Nadeau KC, Li Z, Farzan S, Koestler D, Robbins D, Fei DL, Malipatlolla M, Maecker H, Enelow R, Korrick S, Karagas MR. In utero arsenic exposure and fetal immune repertoire in a US pregnancy cohort. Clinical Immunology 2014;155(2):188-197. |
R834599 (2011) R835442 (2015) R835442 (2016) |
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Oken E, Choi AL, Karagas MR, Marien K, Rheinberger CM, Schoeny R, Sunderland E, Korrick S. Which fish should I eat? Perspectives influencing fish consumption choices. Environmental Health Perspectives 2012;120(6):790-798. |
R834599 (2011) R834599 (2012) R834599 (Final) R834599C001 (2012) R834599C001 (Final) |
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Punshon T, Tappero R, Ricachenevsky FK, Hirschi K, Nakata PA. Contrasting calcium localization and speciation in leaves of the Medicago truncatula mutant cod5 analyzed via synchrotron X-ray techniques. The Plant Journal 2013;76(4):627-633. |
R834599 (Final) R834599C002 (Final) |
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Punshon T, Ricachenevsky FK, Hindt MN, Socha AL, Zuber H. Methodological approaches for using synchrotron X-ray fluorescence (SXRF) imaging as a tool in ionomics: examples from Arabidopsis thaliana. Metallomics 2013;5(9):1133-1145. |
R834599 (Final) R834599C002 (Final) |
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Shi X, Miller S, Mwenda K, Onda A, Rees J, Onega T, Gui J, Karagas M, Demidenko E, Moeschler J. Mapping disease at an approximated individual level using aggregate data: a case study of mapping New Hampshire birth defects. International Journal of Environmental Research and Public Health 2013;10(9):4161-4174. |
R834599 (Final) R834599C001 (Final) R834599C003 (Final) |
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Sunderland EM, Amirbahman A, Burgess NM, Dalziel J, Harding G, Jones SH, Kamai E, Karagas MR, Shi X, Chen CY. Mercury sources and fate in the Gulf of Maine. Environmental Research 2012;119:27-41. |
R834599 (2011) R834599 (2012) R834599C001 (2012) |
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Wilhelm-Benartzi CS, Koestler DC, Karagas MR, Flanagan JM, Christensen BC, Kelsey KT, Marsit CJ, Houseman EA, Brown R. Review of processing and analysis methods for DNA methylation array data. British Journal of Cancer 2013;109(6):1394-1402. |
R834599 (Final) R834599C001 (Final) |
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Yang J, Punshon T, Guerinot ML, Hirschi KD. Plant calcium content: ready to remodel. Nutrients 2012;4(8):1120-1136. |
R834599 (2012) R834599C002 (2012) |
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Supplemental Keywords:
Analytical, arsenic, biology, birth defects, children, community-based, decision making, drinking water, environmental chemistry, EPA Region 1, epidemiology, exposure, food processing, geography, ground water, health effects, heavy metals, human health, immunology, infants, measurement methods, metals, Northeast, population, public good, public policy, risk, sensitive populations, surveys, susceptibility, vulnerability, water, water safety, RFA, Health, Scientific Discipline, INTERNATIONAL COOPERATION, ENVIRONMENTAL MANAGEMENT, HUMAN HEALTH, Exposure, Environmental Chemistry, Biochemistry, Environmental Monitoring, Children's Health, Environmental Policy, Biology, Risk Assessment, birth defects, prenatal exposure, drinking water, perinatal exposure, children's vulnerablity, biological markers, arsenic exposure, dietary exposure, growth & development, developmental disordersRelevant Websites:
Progress and Final Reports:
Original Abstract Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R834599C001 Arsenic and Maternal and Infant Immune Function
R834599C002 Food Borne Exposure to Arsenic During the First Year of Life
R834599C003 An Integrated Geospatial and Epidemiological Study of Associations Between Birth Defects and Arsenic Exposure in New England
R834599C004 Determining How Arsenic (As) Modulates Sonic Hedgehog (Shh) Signaling During Development
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.