Grantee Research Project Results
2012 Progress Report: The Columbia Center for Children’s Environmental Health
EPA Grant Number: R834509Center: The Columbia Center for Children’s Environmental Health
Center Director: Perera, Frederica P.
Title: The Columbia Center for Children’s Environmental Health
Investigators: Perera, Frederica P. , Miller, Rachel L. , Whyatt, Robin M. , Rundle, Andrew , Evans, David , Tang, Deliang , Champagne, Frances , Shepard, Peggy , Rauh, Virginia
Current Investigators: Perera, Frederica P. , Whyatt, Robin M. , Miller, Rachel L. , Evans, David , Rauh, Virginia , Andrews, Howard F. , Champagne, Frances , Rundle, Andrew , Shepard, Peggy
Institution: Columbia University in the City of New York , West Harlem Environmental Action (WE ACT for Environmental Justice)
Current Institution: Columbia University in the City of New York , Resources for the Future , West Harlem Environmental Action (WE ACT for Environmental Justice) , Columbia University Mailman School of Public Health
EPA Project Officer: Callan, Richard
Project Period: September 24, 2009 through September 23, 2014 (Extended to September 23, 2015)
Project Period Covered by this Report: September 24, 2011 through September 23,2012
Project Amount: $3,953,320
RFA: Children's Environmental Health and Disease Prevention Research Centers (with NIEHS) (2009) RFA Text | Recipients Lists
Research Category: Children's Health , Human Health
Objective:
The research theme of the Columbia Center for Childrens Environmental Health (CCCEH) is the role of endocrine (hormone system) disruptors, polycyclic aromatic hydrocarbons (PAH) in air pollution and bisphenol A (BPA), in the development of obesity, metabolic syndrome, and neurodevelopmental disorders in children. Consistent with the goals of EPA and NIEHS, we are conducting three closely linked research projects focused on elucidating the mechanisms by which prenatal and postnatal exposure to PAH and BPA may contribute to these disorders, as expressed in early adolescence and the period just before and after puberty. Our overall hypothesis is that early exposures to these endocrine disruptors are risk factors for the development of potentially serious health problems and that epigenetic mechanisms (heritable changes in gene expression or phenotype caused by mechanisms other than changes in the DNA sequence) are involved in mediating their effects. Continuing our partnership with WE ACT for Environmental Justice, and adding a Pediatric Health Specialist to our team, we will communicate our scientific findings to the participating families, at-risk communities, clinicians, and policymakers so that preventive measures can be taken to protect vulnerable children living in urban areas.
The Center is using molecular epidemiologic approaches as it tracks a longitudinal birth cohort of African-American and Dominican children in Northern Manhattan and the South Bronx. Project 1 (R834509C001), The Role of Endocrine Disruptors in Childhood Obesity, is designed to assess whether exposures to BPA and PAHs during pregnancy and early childhood are associated with obesity and metabolic syndrome among children during early adolescence. Project 2 (R834509C002), The Role of Endocrine Disruptors in Neurodevelopmental Disorders, will assess whether prenatal exposure to the same endocrine disruptors, PAHs and BPA, is associated with adverse neurobehavioral effects among children in the years just before and after puberty. Both Projects 1 and 2 will evaluate the role of exposure-related changes in epigenetics/gene expression in mediating these health effects. Project 3 (R834509C003), The Mechanisms of Endocrine Disruptors in Laboratory Mice, will determine the mechanisms by which prenatal PAHs (inhaled) and BPA (oral) exposure affect child obesity and neurobehavioral development by elucidating the associated molecular changes in DNA methylation and gene expression. The research projects are supported by three cores: Core 1, CCCEH Administration (Admin); Core 2, Data Management, and Statistics (DMS); and Core 3, Community Outreach and Translation (COTC).
R834509C001: Endocrine Disruptors and Obesity Among Inner-City Children
Aim 1: To test whether prenatal and early-life exposures to the endocrine disruptors PAH and BPA predict body size growth trajectories and childhood obesity at age 8-12 years. This will be accomplished by following our ongoing birth cohort to age 8-12 years, measuring height and weight at ages 5, 7, 8-12, body composition at age 7, 8-10, and metabolic syndrome components at age 8-12 years. This work takes advantage of a sophisticated geographic information systems based data on the childrens neighborhoods to control for social (e.g., poverty and socio-demographic composition) and physical factors (e.g., playgrounds, parks, fast-food) likely to predict childhood obesity.
Aim 2: To determine whether differences in the methylation status of key genes involved in adipogenesis (PPARγ2, C/EBPα, C/EBPß, C/EBPδ and DLK1), appetite control (FTO), mediates the association between xenobiotic exposures and childhood obesity outcomes. Methylation of these genes will be measured in cord white blood cell DNA by pyrosequencing.
R834509C002: Endocrine Disruptors, Epigenetic Mechanisms and Neurodevelopment
(1) Determine whether prenatal exposures to the endocrine disruptors, PAH and BPA, are associated with adverse neurobehavioral outcomes in peri-pubertal children, as measured by diagnostic assessment of child psychopathology and cognitive functioning; (2) Determine whether prenatal exposure to PAH or BPA is associated with epigenetic changes in candidate genes, selected as being of a priori interest based on the literature,is associated with endocrine disruption and immune dysregulation, and whether altered methylation/gene expression in these candidates is associated with the neurobehavioral outcomes; and (3) Using GIS, determine the extent to which neighborhood-level conditions contribute to neurobehavioral outcomes and/or moderate the individual-level associations between exposure to PAH or BPA and child neurodevelopment.
R834509C003: Molecular/Disease Consequences of Prenatal BPA, PAH Exposure Across Generations
(1) Examine the consequence of prenatal oral BPA exposure on neurobehavioral, obesity and immune dysfunction in Balb/c mice by determining whether prenatal BPA exposure is associated with abnormal brain cytoarchitecture, impaired social, anxiety-like and cognitive performance, greater adult body weight, body fat composition and organ fat, and immune dysfunction in the adult offspring or grandoffspring; (2) Examine the consequence of prenatal oral BPA exposure for tissue-specific molecular modifications in mice by determining prenatal BPA exposure-induced changes in DNA methylation in genes sensitive to endocrine disruption and immune dysregulation in the brain (hippocampus, hypothalamus, cortex), adipocytes and blood of the prenatally BPA exposed offspring and grandoffspring at gestation day19 and adulthood (postnatal day [PND]60); (3) Examine the consequence of prenatal inhaled PAH exposure at current levels determined to exist in New York Citys Northern Manhattan/South Bronx on neurodevelopment and obesity in Balb/c mice by determining whether prenatal PAH exposure is associated with abnormal brain cytoarchitecture, impaired anxiety-like and cognitive performance, greater body weight through weaning to adulthood, body fat composition and organ fat content in adult offspring and grand offspring; and (4) Examine the consequence of prenatal inhaled PAH exposure for tissue specific molecular modifications (DNA methylation) in mice of genes sensitive to endocrine disruption and immune dysregulation in the brain, adipocytes and blood of the prenatally exposed offspring and grandoffspring at gestation day19 and PND60.
Community Outreach and Translation Core
(1) Engage and expand the Community Advisory and Stakeholder Board; (2) Communicate the Centers research findings through the development of educational materials designed for local residents, community organizations, healthcare providers, and other local stakeholders; (3) In collaboration with the Centers Pediatric Health Specialist, facilitate education of public health and clinical professionals working in low-income communities of color; (4) Disseminate the Centers findings using several communication methods; and (5) Through our partnership with WE ACT, expand the capacity of low-income communities of color to advocate for improved environmental conditions by using the Centers scientific findings and related findings by other investigators.
Progress Summary:
R834509C001: Endocrine Disruptors and Obesity Among Inner-City Children
As of 9/24/12, 362 cohort children at ages 8 to 12 (African American and Dominican) had been screened for eligibility and 344 were found to be eligible. Of these 344 children, 324 were enrolled, 16 have refused enrollment and 4 were pending enrollment.
Maternal exposure to higher levels of polycyclic aromatic hydrocarbons (PAH) during the third trimester of pregnancy was found to be associated with higher BMI Z-score at age 5 and age 7 and with higher fat mass at age 7. There was not association observed between prenatal exposure to PAH and fat free mass at age 7. A journal article on this work was in the American Journal of Epidemiology (Vol 175(11):1163-72, 2012). Once the recruitment has been completed at age 8-12 years old, analyses on the effects of prenatal exposure to PAH throughout cohort followup will be completed.
As part of our work studying the effects of prenatal PAH exposures and childhood obesity, we developed and implemented inverse probability weighting (IPW) and marginal structural models (MSM) based methods to assess and adjust for potential biases occurring due to loss to followup in the cohort. These methods are being deployed across our studies of obesity, asthma and neurodevelopmental outcomes in the Center cohort. The methods were published as an online supplement to our American Journal of Epidemiology paper.
Assays of maternal and child urine samples have been completed for Bisphenol A (BPA) analyses of urinary levels of BP and child body size to age 7 are ongoing. We have completed analyses of sociodemographic correlates of the BPA and the variation snf correlations in BPA levels between the mother and the child, and within the child across urine samples collected at different ages.
We have developed an application for the Pediatric Endocrinology Society to measure additional outcome biomarkers in the stored fasting blood samples we are collecting from the children at age 8 to 12. We proposed to measure biomarkers of metabolic and endocrine regulation (leptin and adiponectin) and biomarkers potentially indicative of excess fat accumulation in the liver (aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma glutamyl transferase (GGT)), a condition known as Non-Alcoholic Fatty Liver Disease (NAFLD). This application is being submitted the week of December 3.
We have applied to the Robert Wood Johnson Healthy Eating Program for funding to conduct research on neighborhood food environments, travel time required to shop for food and the childs diet quality. We have been collecting data on the mothers food shopping patterns, including; the locations of the stores she shops at, how often, what is purchased at the stores and the reasons the stores are used (e.g., convenience, price, freshness). Although the families live in neighborhoods of New York City designated as being under served by healthy food outlets, ~95% of the mothers report shopping at a supermarket or a Wholesale Club Warehouse store. We proposed to study how the quality of the food environment immediately around the residence predicts shopping patterns, travel time for shopping and the childs diet quality.
R834509C002: Endocrine Disruptors, Epigenetic Mechanisms and Neurodevelopment
We previously reported that prenatal exposure to PAH at levels encountered in NYC air adversely affected child IQ scores at 5 years of age (Perera 2009). Multiple regression analyses of IQ test scores (on the more informative Weschler Intelligence Scale for children, WISC) for the same children who were re-tested at ages 7 and 9 found that PAH exposure (also monitored in maternal prenatal air) was significantly inversely associated with full score IQ and working memory at both 7 and 9, and with verbal IQ and processing speed at age 9 (manuscrpts in prep.).In addition, we recently reported results of analyses of behavioral outcomes using both PAH exposure measured both via prenatal air monitoring and maternal and cord adducts measured using the high performance liquid chromatography (HPLC)/ fluorescence method specific to benzo[a]pyrene (B[a]P)-DNA adducts. B[a]P is considered a representative PAH. We found that PAH exposure, whether characterized by personal air monitoring or maternal and cord HPLC adducts, was positively associated with symptoms of Anxious/Depressed and Attention Problems (p value < 0.05, Perera 2012a). These results provide evidence that environmental PAH at levels encountered in NYC air can adversely affect child behavior, especially internalizing problems that could affect school performance.
We analyzed the association between maternal prenatal BPA exposure (dichotomized into high/low groups at the upper quartile) and child behavior at ages 3-4 (assessed via the CBCL), using generalized linear models, adjusting for postnatal BPA exposure and other potential confounders (Perera 2012b). We observed significant interactions (p < 0.05) between prenatal BPA urinary concentrations (adjusting for postnatal BPA exposure and other covariates) and child sex on Emotionally Reactive, Aggressive Behavior, and Internalizing Problems scales of the CBCL. After stratifying on sex, the BPA effects were positive and significant among boys on Emotionally Reactive and Aggressive Behavior and positive and borderline significant (p < 0.1) on Sleep Problems, Withdrawn, Internalizing Problems and Externalizing Problems, indicating that boys with prenatal BPA exposure in the highest concentration quartile had, on average, more reported symptoms of problems in these areas. In contrast, among girls, high BPA exposure was associated with lower scores for Anxious/Depressed and Aggressive Behavior (p < 0.05), and Internalizing Problems (p < 0.1) indicating that girls in the high prenatal BPA exposure group had, on average, fewer reported problems in these areas than girls in the low exposure group. Postnatal BPA urinary concentration alone only had a significant negative effect only on Emotionally Reactive within the entire sample. Comparison of results before and after adjusting for postnatal BPA exposure found the effect estimates to be similar, suggesting that the prenatal period may be a more sensitive window for BPA exposure. This manuscript was published by Environmental Health Perspectives.
Our current battery of neurodevelopmental tests at age 9-11 consists of 9 tests and subtests (CPT-Continuous Performance Test, Childs Memory Scales (5 subtests), NEPSY-II (6 subtests), Edinburgh, Weather Prediction Task, Purdue Pegboard, Mental Rotation Task-Clock Test, Water Level Task and Draw a Spiral Task), 5 clinician rating scales/questionnaires (Childrens Depression Rating Scale, Social Responsiveness Scale, Social Communication Questionnaire, Revised Childrens Manifest Anxiety Scale, and the DuPaul-Barkley ADHD Scale) and a semi-structured diagnostic interview (K-SADS). We have completed the full battery of neurodevelopmental tests on 170 who have reached the age of 9 to date. An additional 13 children have completed the tests but are missing the clinical scales.
In the past several months, we have been examining the association between HPLC cord adducts, psychosocial stress, and IQ. These results are currently being written in a manuscript. We are also examining the association between PAH and IQ at later ages, as well as the association between BPA and various behavior and developmental outcomes.
We have successfully completed pyrosequencing assays to assess the methylation of COX- 2, ERα, CEBPα, PDE4D, and TH. We are currently analyzing the association between prenatal PAH and COX-2 and between prenatal BPA and ERα, CEBPα, PDE4D, and TH, respectively. We are also analyzing associations between the methylation of CpG sites on these genes and neurodevelopmental endpoints, including mental and psychomotor developmental indices (from the Bayley Scales at ages 12, 24, and 36 months), IQ (from the WISC at age 7) and child behavior (from the Child Behavior Check List (CBCL) at ages 3, 5, 7, and 11). We are currently working to optimize the analysis of additional CpG sites on the genes listed above and to develop pyrosequencing assays for the remaining gene candidates (NMDAR2b, CCL17, AhR, INF-γ, and THRβ).
We have also geocoded 841 prenatal addresses from the study area in Northern Manhattan and The Bronx and are continuing the process for later ages (currently 4,089 addresses geocoded). Analysis of relationships between exposures and outcomes at older ages using these data is ongoing.
R834509C003: Molecular/Disease Consequences of Prenatal BPA, PAH Exposure Across Generations
Cohorts of Balb/c mice have been exposed prenatally to low dose BPA (2 μg/kg, 20 μg/kg, 200 μg/kg or tocopherol-stripped corn oil control). In addition, we optimized the experimental exposure of PAHs to pregnant mice. We have submitted our findings to Proceedings of the National Academy of Sciences.
Cohorts of Balb/c mice (n = 80) have been exposed prenatally to PAH vs control air, mated and used in experiments to address aims 3 and 4. Analyses are continuing. DNA methylation analysis for all genes is ongoing.
Community Outreach and Translation Core
CASB Development: In collaboration with WE ACT, CCCEH expanded the CASB to include 17 active members from the community and governmental agencies engaged in advocacy and community awareness of environmental health issuesemphasizing the significance of endocrine disrupting chemicals and child development. The current list of CASB members includes representatives from Autism Speaks, Bronx Borough Presidents Office, Community Health Worker Network of NYC, Dominican Medical Association, East Harlem Asthma Center of Excellence, Harlem Childrens Zone Asthma Initiative, Isabella Geriatric Center, New York Presbyterian Hospital WIC Program, Northern Manhattan Perinatal Partnership, Nos Quedamos and Urban Health Plan, Inc. Since the first CASB meeting on September 17, 2010 the Board decided to meet more frequently in the first year of restructuring. In 2011, we held a total of 6 meetings and plan to meet quarterly throughout 2012.
Health Education: Core staff conducted three focus groups to help inform the development of a tip sheet on reducing exposure to Bisphenol-Aa widely used endocrine disrupter. The finalized tip sheet is tailored for parents in our cohort and the wider Northern Manhattan/South Bronx communities and is printed in Spanish and English. To educate caregivers about health risks to children, COTC staff distributed our materials on BPA, IPM and asthma to local community-based organizations, medical practices and community members. A role-modeling health education project was developed for Center research workers to promote healthy lifestyle changes through personal testimony on how they incorporate tips to reduce environmental health exposures in their daily routine. When participants arrive at the Center they see a picture of their research worker along with two action steps that promote a healthy lifestyle. Currently, Core staff and the CASB are developing a holistic educational campaign which highlights positive messaging to promote environmental health through adoption of healthy habits that reduce exposure risks. The campaign is comprised of six themes, including Green Cleaning, Fresh Air, Eat Fresh, Reduce Stress, Toxic-Free Shopping and Create Change.
Education for Public Health and Clinical Professionals: COTC staff continued meeting with Administrative Core to discuss strategies for presenting scientific research findings at grand rounds, local policy forums, and trainings of local leaders on environmental health risks. On October 25, 2011, the Center and WE ACT held the first of several planned community briefings that invited community leaders, elected officials, and policymakers to engage in dialogue about our research findings. Dr. Frederica Perera discussed health risks associated with prenatal and early exposure to diesel exhaust, pesticides, indoor air allergens, and endocrine disrupting chemicals. WE ACTs Executive Director Ms. Peggy Shepard talked about the current state of policy reform regarding exposures common in our urban communities. Over 30 stakeholders engaged in an active discussion on how to effectively translate Center findings to encourage advocacy and prevention. Attendees included representatives from the Dept. of Health and Mental Hygiene, the Environmental Defense Fund, the Environmental Protection Agency, and other health and community service organizations. COTC staff and Ms. Shepard also presented "A Partnership between CCCEH and WE ACT for Community Outreach and Engagement" at the 2012 Children's Environmental Health Symposium. The COTC Program Coordinator also presented "Identifying Perceptions, Attitudes and Health Behaviors around Bisphenol-A Through Community-Based Focus Groups" at the 2012 Partnerships for Environmental Public Health Conference. Center investigators made 33 presentations to scientific or clinical groups during the year, including presentations by Drs. Perera, Miller, Whyatt, Rauh, Evans, Rundle, Herbstman, Perzanowski, Hopener, Andrews, Orjuela, Just, and Iyer.
Working with the Mailman School of Public Health, COTC staff helped develop and disseminate press releases about Center findings. Dr. Virginia Rauhs paper about Prenatal Exposure to Insecticide Chlorpyrifos Linked to Alterations in Brain Structure and Cognition was featured in over 90 online media outlets, including Scientific American, CNN, FOX News, and ABC News. COTC staff also organized and facilitated interviews with news organizations, including the Wall Street Journal cover story on November 8, 2011 that highlighted the work of CCCEH and director Dr. Frederica Perera. Other media coverage includes a CUMC documentary about air pollution and health, and stories in Business Week, US News and World Report, CNN, NPR, and others. COTC staff distributed 18 Center Updates on new findings to our listserv of over 900 subscribers, created a Facebook page and Twitter account to provide children's environmental health resources, and assisted with the completion of the Centers website redesign.
Capacity Building & Informing Policy: During the year, CCCEH researchers have testified to government agencies. The Center Director testified on "The Role of Prenatal Exposure to Environmental Contaminants in Neurodevelopmental Disorders" at a Congressional briefing attended by more than 50 congressional staff. Additionally, Dr. Herbstman testified to the New York State Assemble Standing Committees on Environmental Conservation and Health to examine the use and effectiveness of flame retardants in children products. WE ACT Executive Director Peggy Shepard and other staff members continue to use Center research findings in their ongoing policy reform efforts. COTC continues to collaborate with WE ACTs initiatives to build community capacity to deal with solid waste management, pests and pesticides, and air quality. On April 28, 2012, WE ACT held a conference entitled Green Home, Safe Home: Saving Money by Going Green to build awareness about these issues in Northern Manhattan.
Future Activities:
R834509C001: Endocrine Disruptors and Obesity Among Inner-City Children
The research is on schedule and we plan to continue the research as described in our grant application.
R834509C002: Endocrine Disruptors, Epigenetic Mechanisms and Neurodevelopment
The research is on schedule and we plan to continue the research as described in our grant application.
R834509C003: Molecular/Disease Consequences of Prenatal BPA, PAH Exposure Across Generations
BPA (aims 1,2): Analysis of the effect of postnatal maternal behavior on all BPA-associated effects (neurobiological and behavioral) is ongoing. Planned experiments include analysis of ER protein levels in the brain, analysis of post-weaning growth trajectories, additional behavioral analysis, assessment of fetal brain and placenta gene expression/DNA methylation, and completion of assessment of target genes. Correspondence between blood/spleen/brain gene expression & DNA methylation will be determined.
PAH (aims 3,4): In the next period, additional mice from multiple batches of PAH exposure will be used to complete the above observations. Brain cytoarchitecture in the hypothalamus, hippocampus and cortex in the adult offspring will be examined histologically. Plus, a few females per treatment group (negative control and PAH exposed) will be mated with adult Balb/c males to test for effects on the F2 generation. Methylation experiments will have been started. Experiments studying high methyl donor diets are planned.
Community Outreach and Translation Core
COTC staff and the CASB will develop a holistic education campaign using healthy literacy and culturally tailored messaging about environmental exposures studied at the Center. COTC will continue to make presentations hosted by CASB member organizations.
Journal Articles: 60 Displayed | Download in RIS Format
| Other center views: | All 104 publications | 62 publications in selected types | All 60 journal articles |
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Albert DA, Begg MD, Andrews HF, Williams SZ, Ward A, Conicella ML, Rauh V, Thompson JL, Papapanou PN. An examination of periodontal treatment, dental care, and pregnancy outcomes in an insured population in the United States. American Journal of Public Health 2011;101(1):151-156. |
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Buckley JP, Engel SM, Braun JM, Whyatt RM, Daniels JL, Mendez MA, Richardson DB, Xu Y, Calafat AM, Wolff MS, Lanphear BP, Herring AH, Rundle AG. Prenatal phthalate exposures and body mass index among 4- to 7-year-old children: a pooled analysis. Epidemiology 2016;27(3):449-458. |
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Choi H, Wang L, Lin X, Spengler JD, Perera FP. Fetal window of vulnerability to airborne polycyclic aromatic hydrocarbons on proportional intrauterine growth restriction. PLoS One 2012;7(4):e35464 (11 pp.). |
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Choi H, Perera FP. Sources of greater fetal vulnerability to airborne polycyclic aromatic hydrocarbons among African Americans. Journal of Epidemiology and Community Health 2012;66(2):121-126. |
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Durham T, Guo J, Cowell W, Riley K, Wang S, Tang D, Perera F, Herbstman J. Prenatal PM2.5 Exposure in Relation to Maternal and Newborn Telomere Length at Delivery. Toxics 23;10(1):13. |
R834509 (Final) R827027 (2002) R832141 (Final) R836154 (Final) |
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Genkinger JM, Stigter L, Jedrychowski W, Huang T-J, Wang S, Roen EL, Majewska R, Kieltyka A, Mroz E, Perera FP. Prenatal polycyclic aromatic hydrocarbon (PAH) exposure, antioxidant levels and behavioral development of children ages 6-9. Environmental Research 2015;140:136-144. |
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Herbstman JB, Tang D, Zhu D, Qu L, Sjodin A, Li Z, Camann D, Perera FP. Prenatal exposure to polycyclic aromatic hydrocarbons, benzo[a]pyrene-DNA adducts, and genomic DNA methylation in cord blood. Environmental Health Perspectives 2012;120(5):733-738. |
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Herbstman JB, Wang S, Perera FP, Lederman SA, Vishnevetsky J, Rundle AG, Hoepner LA, Qu L, Tang D. Predictors and consequences of global DNA methylation in cord blood and at three years. PLoS One 2013;8(9):e72824 (10 pp.). |
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Hoepner LA, Whyatt RM, Just AC, Calafat AM, Perera FP, Rundle AG. Urinary concentrations of bisphenol A in an urban minority birth cohort in New York City, prenatal through age 7 years. Environmental Research 2013;122:38-44. |
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Hoepner LA, Whyatt RM, Widen EM, Hassoun A, Oberfield SE, Mueller NT, Diaz D, Calafat AM, Perera FP, Rundle AG. Bisphenol A and adiposity in an inner-city birth cohort. Environmental Health Perspectives 2016;124(10):1644-1650. |
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Iyer S, Perera F, Zhang B, Chanock S, Wang S, Tang D. Significant interactions between maternal PAH exposure and haplotypes in candidate genes on B[a]P-DNA adducts in a NYC cohort of non-smoking African-American and Dominican mothers and newborns. Carcinogenesis 2014;35(1):69-75. |
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Jung KH, Bernabe K, Moors K, Yan B, Chillrud SN, Whyatt R, Camann D, Kinney PL, Perera FP, Miller RL. Effects of floor level and building type on residential levels of outdoor and indoor polycyclic aromatic hydrocarbons, black carbon, and particulate matter in New York City. Atmosphere 2011;2(2):96-109. |
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Jung KH, Perzanowski M, Rundle A, Moors K, Yan B, Chillrud SN, Whyatt R, Camann D, Perera FP, Miller RL. Polycyclic aromatic hydrocarbon exposure, obesity and childhood asthma in an urban cohort. Environmental Research 2014;128:35-41. |
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Jung KH, Liu B, Lovinsky-Desir S, Yan B, Camann D, Sjodin A, Li Z, Perera F, Kinney P, Chillrud S, Miller RL. Time trends of polycyclic aromatic hydrocarbon exposure in New York City from 2001 to 2012: assessed by repeat air and urine samples. Environmental Research 2014;131:95-103. |
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Jung K, Goowin K, Perzanowski M, Chillrud S, Perera F, Miller R, Lovinsky-Desir S. Personal Exposure to Black Carbon at School and Levels of Fractional Exhaled Nitric Oxide in New York City. Environmental Health Prespectives 2021;129(9). |
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Kundakovic M, Champagne FA. Epigenetic perspective on the developmental effects of bisphenol A. Brain, Behavior, and Immunity 2011;25(6):1084-1093. |
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Kundakovic M, Gudsnuk K, Franks B, Madrid J, Miller RL, Perera FA. Champagne FA. Sex-specific epigenetic disruption and behavioral changes following low-dose in utero bisphenol A exposure. Proceedings of the National Academy of Sciences of the United States of America 2013;110(24):9956-9961. |
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Kundakovic M, Gudsnuk K, Herbstman JB, Tang D, Perera FP, Champagne FA. DNA methylation of BDNF as a biomarker of early-life adversity. Proceedings of the National Academy of Sciences of the United States of America 2015;112(22):6807-6813. |
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Lovasi GS, Quinn JW, Rauh VA, Perera FP, Andrews HF, Garfinkel R, Hoepner L, Whyatt R, Rundle A. Chlorpyrifos exposure and urban residential environment characteristics as determinants of early childhood neurodevelopment. American Journal of Public Health 2011;101(1):63-70. |
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Lovasi GS, O’Neil-Dunne JPM, Lu JWT, Sheehan D, Perzanowski MS, MacFaden SW, King KL, Matte T, Miller RL, Hoepner LA, Perera FP, Rundle A. Urban tree canopy and asthma, wheeze, rhinitis, and allergic sensitization to tree pollen in a New York City birth cohort. Environmental Health Perspectives 2013;121(4):494-500. |
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Lovasi GS, Eldred-Skemp N, Quinn JW, Chang HW, Rauh VA, Rundle A, Orjuela MA, Perera FP. Neighborhood social context and individual polycyclic aromatic hydrocarbon exposures associated with child cognitive test scores. Journal of Child and Family Studies 2014;23(5):785-799. |
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Lovinsky-Desir S, Miller RL. Epigenetics, asthma, and allergic diseases:a review of latest advancements. Current Allergy and Asthma Reports 2012;12(3):211-220. |
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Maresca MM, Hoepner LA, Hassoun A, Oberfield SE, Mooney SJ, Calafat AM, Ramirez J, Freyer G, Perera FP, Whyatt RM, Rundle AG. Prenatal exposure to phthalates and childhood body size in an urban cohort. Environmental Health Perspectives 2015 June 12 [Epub ahead of print], doi:10.1289/ehp.1408750. |
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Maresca MM, Hoepner LA, Hassoun A, Oberfield SE, Mooney SJ, Calafat AM, Ramirez J, Freyer G, Perera FP, Whyatt RM, Rundle AG. Prenatal exposure to phthalates and childhood body size in an urban cohort. Environmental Health Perspectives 2016;124(4):514-520. |
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Margolis AE, Herbstman JB, Davis KS, Thomas VK, Tang D, Wang Y, Wang S, Perera FP, Peterson BS, Rauh VA. Longitudinal effects of prenatal exposure to air pollutants on self-regulatory capacities and social competence. Journal of Child Psychology and Psychiatry 2016;57(7):851-860. |
R834509 (Final) R836154 (2017) |
Exit Exit |
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Miller RL, Garfinkel R, Lendor C, Hoepner L, Li Z, Romanoff L, Sjodin A, Needham L, Perera FP, Whyatt RM. Polycyclic aromatic hydrocarbon metabolite levels and pediatric allergy and asthma in an inner-city cohort. Pediatric Allergy and Immunology 2010;21(2 Pt 1):260-267. |
R834509 (2011) R834509 (Final) R832096 (Final) R832141 (2007) R832141 (Final) |
Exit |
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Mueller NT, Whyatt R, Hoepner L, Oberfield S, Dominguez-Bello MG, Widen EM, Hassoun A, Perera F, Rundle A. Prenatal exposure to antibiotics, cesarean section and risk of childhood obesity. International Journal of Obesity 2015;39(4):665-670. |
R834509 (2014) R834509 (Final) R834509C001 (Final) |
Exit |
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Nobel KG, Fifer WP, Rauh VA, Nomura Y, Andrews HF. Academic achievement varies with gestational age among children born at term. Pediatrics 2012;130(2):e257-e264. |
R834509 (Final) |
Exit Exit |
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Orjuela MA, Liu X, Warburton D, Siebert AL, Cujar C, Tang D, Jobanputra V, Perera FP. Prenatal PAH exposure is associated with chromosome-specific aberrations in cord blood. Mutation Research 2010;703(2):108-114. |
R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit Exit Exit |
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Orjuela MA, Liu X, Miller RL, Warburton D, Tang D, Jobanputra V, Hoepner L, Suen IH, Diaz-Carreno S, Li Z, Sjodin A, Perera FP. Urinary naphthol metabolites and chromosomal aberrations in 5-year-old children. Cancer Epidemiology, Biomarkers & Prevention 2012;21(7):1191-1202. |
R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) R832096 (Final) |
Exit Exit Exit |
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Patel MM, Hoepner L, Garfinkel R, Chillrud S, Reyes A, Quinn JW, Perera F, Miller RL. Ambient metals, elemental carbon, and wheeze and cough in New York City children through 24 months of age. American Journal of Respiratory and Critical Care Medicine 2009;180(11):1107-1113. |
R834509 (2011) R834509 (Final) R827027 (2002) R832141 (2007) R832141 (Final) |
Exit Exit Exit |
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Perera FP, Li Z, Whyatt R, Hoepner L, Wang S, Camann D, Rauh V. Prenatal airborne polycyclic aromatic hydrocarbon exposure and child IQ at age 5 years. Pediatrics 2009;124(2):e195-e202. |
R834509 (2014) R834509 (Final) R834509C002 (Final) R832141 (Final) |
Exit |
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Perera FP, Wang S, Vishnevetsky J, Zhang B, Cole KJ, Tang D, Rauh V, Phillips DH. Polycyclic aromatic hydrocarbons-aromatic DNA adducts in cord blood and behavior scores in New York City children. Environmental Health Perspectives 2011;119(8):1176-1181. |
R834509 (2011) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) |
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Perera FP, Tang D, Wang S, Vishnevetsky J, Zhang B, Diaz D, Camann D, Rauh V. Prenatal polycyclic aromatic hydrocarbon (PAH) exposure and child behavior at age 6-7 years. Environmental Health Perspectives 2012;120(6):921-926. |
R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (2012) R834509C002 (Final) |
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Perera FP, Chang HW, Tang D, Roen EL, Herbstman J, Margolis A, Huang TJ, Miller RL, Wang S, Rauh V. Early-life exposure to polycyclic aromatic hydrocarbons and ADHD behavior problems. PLoS One 2014;9(11):e111670 (9 pp.). |
R834509 (2014) R834509 (Final) R834509C002 (Final) R832096 (Final) |
Exit Exit |
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Perera F, Herbstman J. Prenatal environmental exposures, epigenetics, and disease. Reproductive Toxicology 2011;31(3):363-373. |
R834509 (2011) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit Exit Exit |
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Perera F, Vishnevetsky J, Herbstman JB, Calafat AM, Xiong W, Rauh V, Wang S. Prenatal bisphenol A exposure and child behavior in an inner-city cohort. Environmental Health Perspectives 2012;120(8):1190-1194. |
R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (2012) R834509C002 (Final) |
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Perera F, Weiland K, Neidell M, Wang S. Prenatal exposure to airborne polycyclic aromatic hydrocarbons and IQ:estimated benefit of pollution reduction. Journal of Public Health Policy 2014;35(3):327-336. |
R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit |
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Perera F, Phillips DH, Wang Y, Roen E, Herbstman J, Rauh V, Wang S, Tang D. Prenatal exposure to polycyclic aromatic hydrocarbons/aromatics, BDNF and child development. Environmental Research 2015;142:602-608. |
R834509 (Final) R834509C002 (Final) |
Exit Exit Exit |
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Peterson BS, Rauh VA, Bansal R, Hao X, Toth Z, Nati G, Walsh K, Miller RL, Arias F, Semanek D, Perera F. Effects of prenatal exposure to air pollutants (polycyclic aromatic hydrocarbons) on the development of brain white matter, cognition, and behavior in later childhood. JAMA Psychiatry 2015;72(6):531-540. |
R834509 (Final) R834509C002 (Final) R832096 (Final) |
Exit |
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Rauh VA, Horton MK, Miller RL, Whyatt RM, Perera F. Neonatology and the environment:impact of early exposure to airborne environmental toxicants on infant and child neurodevelopment. Neoreviews 2010;11(7):363-369. |
R834509 (2011) R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) R834509C003 (2012) R834509C003 (Final) |
Exit Exit |
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Roen EL, Wang Y, Calafat AM, Wang S, Margolis A, Herbstman J, Hoepner LA, Rauh V, Perera FP. Bisphenol A exposure and behavioral problems among inner city children at 7-9 years of age. Environmental Research 2015;142:739-745. |
R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit Exit Exit |
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Rundle AG, Gallagher D, Herbstman JB, Goldsmith J, Holmes D, Hassoun A, Oberfield S, Miller RL, Andrews H, Widen EM, Hoepner LA. Prenatal exposure to airborne polycyclic aromatic hydrocarbons and childhood growth trajectories from age 5-14 years. Environmental research 2019;177:108595. |
R834509 (Final) R827027 (2002) R832141 (Final) |
Exit Exit |
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Rundle A, Hoepner L, Hassoun A, Oberfield S, Freyer G, Holmes D, Reyes M, Quinn J, Camann D, Perera F, Whyatt R. Association of childhood obesity with maternal exposure to ambient air polycyclic aromatic hydrocarbons during pregnancy. American Journal of Epidemiology 2012;175(11):1163-1172. |
R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C001 (2012) R834509C001 (Final) |
Exit Exit Exit |
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Rundle A, Rauh VA, Quinn J, Lovasi G, Transande L, Susser E, Andrews HF. Use of community-level data in the National Children’s Study to establish the representativeness of segment selection in the Queens Vanguard Site. International Journal of Health Geographics 2012;11:18 (11 pp.). |
R834509 (Final) |
Exit Exit |
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Tang WY, Levin L, Talaska G, Cheung YY, Herbstman J, Tang D, Miller RL, Perera F, Ho SM. Maternal exposure to polycyclic aromatic hydrocarbons and 5'-CpG methylation of interferon-γ in cord white blood cells. Environmental Health Perspectives 2012;120(8):1195-1200. |
R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (2012) R834509C002 (Final) |
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Vishnevetsky J, Tang D, Chang HW, Roen EL, Wang Y, Rauh V, Wang S, Miller RL, Herbstman J, Perera FP. Combined effects of prenatal polycyclic aromatic hydrocarbons and material hardship on child IQ. Neurotoxicology and Teratology 2015;49:74-80. |
R834509 (2014) R834509 (Final) R834509C002 (Final) R832096 (Final) |
Exit Exit Exit |
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Wang S, Chanock S, Tang D, Li Z, Edwards S, Jedrychowski W, Perera FP. Effect of gene-environment interactions on mental development in African American, Dominican, and Caucasian mothers and newborns. Annals of Human Genetics 2010;74(1):46-56. |
R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) R832141 (2007) R832141 (Final) |
Exit Exit Exit |
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Wang S, Yu Z, Miller RL, Tang D, Perera FP. Methods for detecting interactions between imprinted genes and environmental exposures using birth cohort designs with mother-offspring pairs. Human Heredity 2011;71(3):196-208. |
R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit Exit |
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Wang S, Xiong W, Ma W, Chanock S, Jedrychowski W, Wu R, Perera FP. Gene-environment interactions on growth trajectories. Genetic Epidemiology 2012;36(3):206-213. |
R834509 (2012) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (2012) R834509C002 (Final) |
Exit Exit |
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Wang Y, Perera F, Guo J, Riley K, Durham T, Ross Z, Ananth C, Baccarelli A, Wang S, Herbstman J. A methodological pipeline to generate an epigenetic marker of prenatal exposure to air pollution indicators. Epigenetics 2021;1-9. |
R834509 (Final) R827027 (2002) R832141 (Final) |
Exit |
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Weiland K, Neidell M, Rauh V, Perera F. Cost of developmental delay from prenatal exposure to airborne polycyclic aromatic hydrocarbons. Journal of Health Care for the Poor and Underserved 2011;22(1):320-329. |
R834509 (2011) R834509 (2013) R834509 (2014) R834509 (Final) R834509C002 (Final) |
Exit Exit |
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Widen EM, Whyatt RM, Hoepner LA, Ramirez-Carvey J, Oberfield SE, Hassoun A, Perera FP, Gallagher D, Rundle AG. Excessive gestational weight gain is associated with long-term body fat and weight retention at 7 y postpartum in African American and Dominican mothers with underweight, normal, and overweight prepregnancy BMI. American Journal of Clinical Nutrition 2015;102(6):1460-1467. |
R834509 (Final) R834509C001 (Final) |
Exit Exit |
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Widen EM, Whyatt RM, Hoepner LA, Mueller NT, Ramirez-Carvey J, Oberfield SE, Hassoun A, Perera FP, Gallagher D, Rundle AG. Gestational weight gain and obesity, adiposity and body size in African-American and Dominican children in the Bronx and Northern Manhattan. Maternal and Child Nutrition 2016;12(4):918-928. |
R834509 (Final) |
Exit Exit |
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Widen E, Burns N, Daniels M, Backlund G, Rickman R, Foster S, Nichols A, Hoepner L, Kinsey E, Ramireaz-Carvey J. Gestational weight change and childhood body composition trajectories from pregnancy to early adolescence. Obesity 10;. |
R834509 (Final) R827027 (2002) R832141 (Final) |
Exit Exit |
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Widen E, Nichols A, Kahn L, Factor-Livak P, Insel B, Hoepner L, Dube S, Rauh V, Perera F, Rundel A. Prepregnancy obesity is associated with cognitive outcomes in boys in a low-income, multiethnic birth cohort. BMC Pediatrics 2019;19(1):507. |
R834509 (Final) R827027 (2002) |
Exit Exit |
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Witherspoon NO, Trousdale K, Bearer CF, Miller RL. The public health and policy implications of epigenetics and pediatric health research. Environmental Health Perspectives 2012;120(10):a380-a381. |
R834509 (2012) R834509 (2013) R834509C003 (2012) |
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Yan Z, Zhang H, Maher C, Arteaga-Solis E, Champagne FA, Wu L, McDonald JD, Yan B, Schwartz GJ, Miller RL. Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice. PLoS One 2014;9(10):e110706 (15 pp.). |
R834509 (2014) R834509 (Final) R834509C003 (Final) |
Exit Exit |
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Zeinomar N, Grant-Alfieri A, Burke K, de Hoz M, Tehranifar P, Walker D, Morton T, Shepard P, Herbstman J, Miller R, Pera F, Terry M. Cancer Risk Reduction Through Education of Adolescents:Development of a Tailored Cancer Risk-Reduction Educational Tool. Journal of Cancer Education 2021;. |
R834509 (Final) R827027 (2002) R832141 (Final) |
Exit Exit |
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Widen EM, Whyatt RM, Hoepner LA, Mueller NT, Ramirez‐Carvey J, Oberfield SE, Hassoun A, Perera FP, Gallagher D, Rundle AG. Gestational weight gain and obesity, adiposity and body size in African-American and Dominican children in the Bronx and Northern Manhattan. Maternal & Child Nutrition 2016;12(4):918-928. |
R834509C001 (Final) R836154 (2017) |
Exit |
Supplemental Keywords:
Bisphenol A, polycyclic aromatic hydrocarbons, obesity, neurodevelopment, children, miceRelevant Websites:
Progress and Final Reports:
Original Abstract Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R834509C001 The Role of Endocrine Disruptors in Childhood Obesity
R834509C002 The Role of Endocrine Disruptors in Neurodevelopmental Disorders
R834509C003 The Mechanisms of Endocrine Disruptors in Laboratory Mice
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.