2004 Progress Report: Metals Neurotoxicity Research Project

EPA Grant Number: R831725C004
Subproject: this is subproject number 004 , established and managed by the Center Director under grant R831725
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: Harvard Center for Children’s Environmental Health and Disease Prevention Research
Center Director: Hu, Howard
Title: Metals Neurotoxicity Research Project
Investigators: Maher, Tim , Weisskopf, Marc
Institution: Harvard University
EPA Project Officer: Callan, Richard
Project Period: June 1, 2004 through May 31, 2009 (Extended to May 31, 2011)
Project Period Covered by this Report: June 1, 2004 through May 31, 2005
RFA: Centers for Children's Environmental Health and Disease Prevention Research (2003) RFA Text |  Recipients Lists
Research Category: Human Health , Children's Health

Objective:

The objectives of this research project are to:  (1) examine the effect of pre- and neonatal exposure to metals on neurochemical changes and neurobehavioral outcomes in rats; and (2) explore the potential modifying effect of stress on metals neurotoxicity.

Progress Summary:

Progress on Project 4 has been hindered by the delay in getting the subcontract to the Massachusetts College of Pharmacy finalized.  We have, however, recently received the behavioral chambers for the Fixed-Ratio Waiting for Reward paradigm and are working out the details of the software programming.  The Repeated Acquisition and Performance Chamber for the other behavioral paradigm currently is being designed and built.  We are validating the High Performance Liquid Chromatography (HPLC) assays for dopamine, its metabolites, and glutamate.  We also are finalizing the details of the assay for 8-OH-2’-dG and validating the assay. 

Future Activities:

Pilot testing of animals should begin shortly.  As soon as the subcontract is finalized, we will be able to purchase the autosampler for HPLC analyses.  We anticipate significant progress in the months to come.

Journal Articles:

No journal articles submitted with this report: View all 12 publications for this subproject

Supplemental Keywords:

children, Native American, tribal, mixtures, lead, PBPK, community, Superfund, intervention, environmental management,, RFA, Health, Scientific Discipline, INTERNATIONAL COOPERATION, ENVIRONMENTAL MANAGEMENT, Waste, Environmental Chemistry, Health Risk Assessment, Hazardous Waste, Biochemistry, Children's Health, Hazardous, Risk Assessment, community-based intervention, fate and transport , developmental toxicity, Human Health Risk Assessment, neurodevelopmental toxicity, children's environmental health, mining waste, metal wastes, metals, human health risk, metal contamination

Relevant Websites:

http://www.hsph.harvard.edu/niehs/children Exit

Progress and Final Reports:

Original Abstract
  • 2005 Progress Report
  • 2006
  • 2007 Progress Report
  • 2008 Progress Report
  • 2009
  • Final

  • Main Center Abstract and Reports:

    R831725    Harvard Center for Children’s Environmental Health and Disease Prevention Research

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R831725C001 Metals, Nutrition, and Stress in Child Development
    R831725C002 Exposure Assessment of Children and Metals in Mining Waste: Composition, Environmental Transport, and Exposure Patterns
    R831725C003 Manganese, Iron, Cadmium, and Lead Transport from the Environment to Critical Organs During Gestation and Early Development in a Rat Model
    R831725C004 Metals Neurotoxicity Research Project