Grantee Research Project Results
2011 Progress Report: Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
EPA Grant Number: R832415C005Subproject: this is subproject number 005 , established and managed by the Center Director under grant R832415
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Rochester PM Center
Center Director: Oberdörster, Günter
Title: Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
Investigators: Finkelstein, Jacob N. , Prather, Kimberly A. , Phipps, Richard , Oakes, David , Rahman, Arshad
Current Investigators: Finkelstein, Jacob N. , Prather, Kimberly A. , Rahman, Arshad , Oakes, David , Phipps, Richard
Institution: University of Rochester , University of California - San Diego
EPA Project Officer: Chung, Serena
Project Period: October 1, 2005 through September 30, 2010 (Extended to September 30, 2012)
Project Period Covered by this Report: October 1, 2010 through September 30,2011
RFA: Particulate Matter Research Centers (2004) RFA Text | Recipients Lists
Research Category: Human Health , Air
Objective:
The experiments proposed within this project are designed to address specific mechanistic hypotheses regarding the interactions between inhaled ultrafine particles and specific pulmonary cell populations. In vitro experiments are best utilized to provide a mechanistic link and biological plausibility for the whole animal and controlled clinical (human) exposures, described in the other programs of this Particle Center. In some circumstances they can be used to screen materials for acute toxicity or provide an initial ranking of response. In our original proposal we had suggested a focus on identifying specific responses and mechanisms that may be involved in the enhanced susceptibility of diabetic. We continue to explore this in the context of the oxidant stress hypothesis of PM induced effects.
Progress Summary:
Sample # | Start | Stop | Volume syringe (mL) | total minutes | Estimated Concentraion (ug/mL) |
---|---|---|---|---|---|
B01 | 05/10/11 19:23 | 05/11/11 06:10 | 14.0 | 647 | 13.1 |
B02 | 05/11/11 06:20 | 05/11/11 12:15 | 13.0 | 647 | 9.5 |
B03 | 05/11/11 12:21 | 05/11/11 17:42 | 11.0 | 321 | 10.4 |
B04 | 05/11/11 17:51 | 05/12/11 06:05 | 15.0 | 734 | 12.8 |
B05 | 05/12/11 06:14 | 05/12/11 12:01 | 12.0 | 347 | 11.8 |
B06 | 05/12/11 12:03 | 05/12/11 23:32 | 18.0 | 689 | 10.0 |
B07 | 05/12/11 23:40 | 05/13/11 09:30 | 17.0 | 590 | 9.4 |
B08 | 05/13/11 09:35 | 05/13/11 16:34 | 14.0 | 419 | 11.3 |
B09 | 05/13/11 16:45 | 05/13/11 22:36 | 11.0 | 351 | 11.5 |
B10 | 05/13/11 22:39 | 05/14/11 10:41 | 21.5 | 722 | 9.7 |
B11 | 05/14/11 10:47 | 05/14/11 17:08 | 13.0 | 381 | 8.6 |
B12 | 05/14/11 17:15 | 05/14/11 22:42 | 10.5 | 327 | 9.4 |
B13 | 05/14/11 22:45 | 05/15/11 09:23 | 21.0 | 638 | 4.8 |
B14 | 05/15/11 09:30 | 05/15/11 14:27 | 11 | 297 | 2.7 |
B15 | 05/15/11 14:31 | 05/15/11 21:42 | 8.0 | 431 | 3.3 |
B16 | 05/15/11 24:46 | 05/16/11 08:50 | 10.0 | 664 | 8.7 |
Figure 1. Response of A549 cells to collected ambient samples. Results are
compared to varying concentrations of TiO2 (right bars)
Journal Articles on this Report : 10 Displayed | Download in RIS Format
Other subproject views: | All 13 publications | 11 publications in selected types | All 10 journal articles |
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Other center views: | All 191 publications | 157 publications in selected types | All 144 journal articles |
Type | Citation | ||
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Elder ACP, Gelein R, Azadniv M, Frampton M, Finkelstein J, Oberdorster G. Systemic effects of inhaled ultrafine particles in two compromised, aged rat strains. Inhalation Toxicology 2004;16(6-7):461-471. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C003 (2011) R832415C004 (2011) R832415C005 (2011) R826784 (Final) R827354 (Final) R827354C003 (Final) R827354C004 (2003) R827354C004 (Final) R827354C005 (Final) R828046 (Final) |
Exit |
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Elder ACP, Gelein R, Oberdorster G, Finkelstein J, Notter R, Wang Z. Efficient depletion of alveolar macrophages using intratracheally inhaled aerosols of liposome-encapsulated clodronate. Experimental Lung Research 2004;30(2):105-120. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C004 (2011) R832415C005 (2011) R827354 (Final) R827354C003 (Final) R827354C004 (2003) R827354C004 (Final) R827354C005 (Final) |
Exit |
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Elder A, Gelein R, Finkelstein J, Phipps R, Frampton M, Utell M, Kittelson DB, Watts WF, Hopke P, Jeong C-H, Kim E, Liu W, Zhao W, Zhuo L, Vincent R, Kumarathasan P, Oberdorster G. On-road exposure to highway aerosols. 2. Exposures of aged, compromised rats. Inhalation Toxicology 2004;16(Suppl 1):41-53. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C003 (2011) R832415C004 (2011) R832415C005 (2011) R827354 (Final) R827354C003 (Final) R827354C004 (2003) R827354C004 (Final) R827354C005 (Final) R828046 (Final) |
Exit |
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Elder A, Johnston C, Gelein R, Finkelstein J, Wang Z, Notter R, Oberdorster G. Lung inflammation induced by endotoxin is enhanced in rats depleted of alveolar macrophages with aerosolized clodronate. Experimental Lung Research 2005;31(6):527-546. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C004 (2011) R832415C005 (2011) R827354 (Final) R827354C004 (Final) R827354C005 (Final) R828046 (Final) |
Exit |
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Elder A, Gelein R, Silva V, Feikert T, Opanashuk L, Carter J, Potter R, Maynard A, Ito Y, Finkelstein J, Oberdorster G. Translocation of inhaled ultrafine manganese oxide particles to the central nervous system. Environmental Health Perspectives 2006;114(8):1172-1178. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C004 (2011) R832415C005 (2011) R827354 (Final) R827354C004 (Final) R827354C005 (Final) |
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Fanning EW, Froines JR, Utell MJ, Lippmann M, Oberdorster G, Frampton M, Godleski J, Larson TV. Particulate Matter (PM) Research Centers (1999-2005) and the role of interdisciplinary center-based research. Environmental Health Perspectives 2009;117(2):167-174. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C003 (2011) R832415C004 (2011) R832415C005 (2011) R827351 (Final) R827352 (Final) R827353 (Final) R827354 (Final) R827355 (Final) R832416 (2009) R832416C003 (2009) |
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Han X, Gelein R, Corson N, Wade-Mercer P, Jiang J, Biswas P, Finkelstein JN, Elder A, Oberdorster G. Validation of an LDH assay for assessing nanoparticle toxicity. Toxicology 2011;287(1-3):99-104. |
R832415 (2011) R832415 (Final) R832415C004 (2011) R832415C005 (2011) |
Exit Exit Exit |
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Rushton EK, Jiang J, Leonard SS, Eberly S, Castranova V, Biswas P, Elder A, Han X, Gelein R, Finkelstein J, Oberdorster G. Concept of assessing nanoparticle hazards considering nanoparticle dosemetric and chemical/biological response metrics. Journal of Toxicology and Environmental Health, Part A 2010;73(5-6):445-461. |
R832415 (2010) R832415 (2011) R832415 (Final) R832415C004 (2010) R832415C004 (2011) R832415C005 (2010) R832415C005 (2011) |
Exit |
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Singal M, Finkelstein JN. Use of indicator cell lines for determining inflammatory gene changes and screening the inflammatory potential of particulate and non-particulate stimuli. Inhalation Toxicology 2005;17(9):415-425. |
R832415 (2010) R832415 (Final) R832415C005 (2011) R827354 (Final) R827354C005 (Final) |
Exit |
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Singal M, Finkelstein JN. Amorphous silica particles promote inflammatory gene expression through the redox sensitive transcription factor, AP-1, in alveolar epithelial cells. Experimental Lung Research 2005;31(6):581-597. |
R832415 (2010) R832415 (Final) R832415C005 (2011) R827354 (Final) R827354C005 (Final) |
Exit |
Supplemental Keywords:
RFA, Scientific Discipline, Health, PHYSICAL ASPECTS, Air, particulate matter, Genetics, Health Risk Assessment, Risk Assessments, Physical Processes, Biology, altered gene expression, atmospheric particulate matter, long term exposure, atmospheric particles, airway disease, exposure, ambient particle health effects, human exposure, atmospheric aerosol particles, PM, aersol particlesProgress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R832415 Rochester PM Center Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R832415C001 Characterization and Source Apportionment
R832415C002 Epidemiological Studies on Extra Pulmonary Effects of Fresh and Aged Urban Aerosols from Different Sources
R832415C003 Human Clinical Studies of Concentrated Ambient Ultrafine and Fine Particles
R832415C004 Animal models: Cardiovascular Disease, CNS Injury and Ultrafine Particle Biokinetics
R832415C005 Ultrafine Particle Cell Interactions In Vitro: Molecular Mechanisms Leading To Altered Gene Expression in Relation to Particle Composition
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- Final Report
- 2010 Progress Report
- 2009 Progress Report
- 2008 Progress Report
- 2007 Progress Report
- 2006 Progress Report
- Original Abstract
10 journal articles for this subproject
Main Center: R832415
191 publications for this center
144 journal articles for this center