Grantee Research Project Results
Project 4 -- Transport and Fate Particles
EPA Grant Number: R832414C004Subproject: this is subproject number 004 , established and managed by the Center Director under grant R832414
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: UC Davis Center for Children's Environmental Health and Disease Prevention
Center Director: Van de Water, Judith
Title: Project 4 -- Transport and Fate Particles
Investigators: Wilson, Dennis , Louie, Angelique
Institution: University of California - Davis
EPA Project Officer: Chung, Serena
Project Period: October 1, 2005 through September 30, 2010 (Extended to September 30, 2011)
RFA: Particulate Matter Research Centers (2004) RFA Text | Recipients Lists
Research Category: Human Health , Air
Objective:
1) To characterize the time course, tissue distribution, and mechanisms of particulate matter (PM) accumulation in the systemic circulation and target organs. 2) To evaluate the effects of size and surface-fixed charge on this process. 3) To determine how altered lung structure affects systemic particle distribution.
Approach:
Ultrafine particulates are transported into the systemic circulation and target organs by mechanisms that have not been characterized. Key questions include whether particulates in the ultrafine range behave differently from larger particles and whether particle composition, especially surface charge, affects systemic absorption. Experiments in this project will address the mechanisms of transport across the pulmonary epithelial barrier, the time course and target tissues for the distribution of particulates, likely means of intravascular transport, mechanisms of particulate interaction with the vascular wall, and the potential for endothelial cell mediated transport into tissues. We will perform in vivo exposures to particles of varied size and surface-fixed charge. These particulates will be composed of materials traceable by microimaging techniques in real time combined; particles will also be histologically quantitated within target tissues. We also propose in vitro experiments to evaluate potential routes of passive or facilitated transport across epithelial and endothelial cell monolayers. Finally we will determine whether animals with lung structure compromised by postnatal oxidant air pollutant exposure have increased systemic circulation of ultrafine particulates and examine the effect of acute oxidant exposure in adult animals on the transport of particles into the systemic circulation.
Expected Results:
These experiments use size and surface-fixed charge defined ultrafine particulates to provide baseline information on the time course and extent of their systemic absorption. Understanding the nature of particle transport in blood will be important for recognizing the likelihood and potential mechanisms for interaction with tissues. Combining microimaging of whole animals in real time with quantitative histologic evaluation of tissue distribution should provide insight into the time course and nature of potential biological responses. The high resolution microscopy and reconstruction techniques to be used in these experiments will not only distinguish whether particles move between or through cells and airway or vessels walls but, in combination with inhibitor studies, whether this is an active or passive process, thereby providing insight into the responsible biologic processes.
Publications and Presentations:
Publications have been submitted on this subproject: View all 3 publications for this subproject | View all 128 publications for this centerJournal Articles:
Journal Articles have been submitted on this subproject: View all 2 journal articles for this subproject | View all 64 journal articles for this centerSupplemental Keywords:
ambient air, ozone, exposure, health effects, human health, metabolism, sensitive populations, infants, children, PAH, metals, oxidants, agriculture, transportation,, RFA, Health, Air, particulate matter, Risk Assessments, toxicology, long term exposure, lung disease, epidemiological studies, PMProgress and Final Reports:
Main Center Abstract and Reports:
R832414 UC Davis Center for Children's Environmental Health and Disease Prevention Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R832414C001 Project 1 -- Pulmonary Metabolic Response
R832414C002 Endothelial Cell Responses to PM—In Vitro and In Vivo
R832414C003 Project 3 -- Inhalation Exposure Assessment of San Joaquin Valley Aerosol
R832414C004 Project 4 -- Transport and Fate Particles
R832414C005 Project 5 -- Architecture Development and Particle Deposition
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
- Final Report
- 2010 Progress Report
- 2009 Progress Report
- 2008 Progress Report
- 2007 Progress Report
- 2006 Progress Report
2 journal articles for this subproject
Main Center: R832414
128 publications for this center
64 journal articles for this center