Grantee Research Project Results
2016 Progress Report: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse
EPA Grant Number: R835826Title: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse
Investigators: Nguyen, Thanh (Helen) H. , Shisler, Joanna L , Guest, Jeremy S
Institution: University of Illinois Urbana-Champaign
EPA Project Officer: Spatz, Kyle
Project Period: September 1, 2015 through August 31, 2018 (Extended to August 31, 2020)
Project Period Covered by this Report: September 1, 2015 through August 31,2016
Project Amount: $750,000
RFA: Human and Ecological Health Impacts Associated with Water Reuse and Conservation Practices (2014) RFA Text | Recipients Lists
Research Category: Human Health , Water
Objective:
1. Determine the molecular mechanisms responsible for virus inactivation;
2. Determine factors required for effective virus inactivation by natural sunlight and UVC; and
3. Develop pond and UVC design guidelines to achieve reliable virus inactivation and elucidate trade-offs across and within dimensions of sustainability.
Progress Summary:
- We determined the mechanism responsible for human rotavirus Wa inactivation. We found that only the inhibition of viral RNA synthesis positively correlated with a loss of rotavirus infectivity. In addition, the decrease in RNA synthesis was responsible for approximately half of the decrease in infectivity, suggesting that other mechanisms, including post-translational, contribute to inactivation.
- We determined the factors required for effective virus inactivation by natural sunlight. We collected wastewater from three sources: secondary effluent from a municipal wastewater treatment plant, a wastewater pond of an animal farming facility, and effluent from a pond system treating municipal wastewater. Using five different rotavirus strains (RVs), we found that RVs had various susceptibilities to sunlight disinfection. For instance, the human RV strains were more susceptible to the endogenous inactivation than the exogenous inactivation. In contrast, the porcine RV (OSU) was more susceptible to exogenous inactivation
Future Activities:
1. Determine the molecular mechanisms responsible for virus solar inactivation of two human strains and one porcine rotavirus;
2. Determine factors required for effective virus inactivation by natural sunlight and UVC using three strains of rotavirus; and 3. Develop pond and UVC design guidelines to achieve reliable virus inactivation and elucidate tradeoffs across and within dimensions of sustainability.
Journal Articles on this Report : 3 Displayed | Download in RIS Format
| Other project views: | All 27 publications | 11 publications in selected types | All 11 journal articles |
|---|
| Type | Citation | ||
|---|---|---|---|
|
|
Feng Z, Lu R, Yuan B, Zhou Z, Wu Q, Nguyen TH. Influence of solution chemistry on the inactivation of particle-associated viruses by UV irradiation. Colloids and Surfaces B: Biointerfaces 2016;148:622–628. |
R835826 (2016) R835826 (2017) R835826 (2019) R835826 (Final) |
Exit Exit Exit |
|
|
Fuzawa M, Ku K-M, Palma-Salgado SP, Nagasaka K, Feng H, Juvik JA, Sano D, Shisler JL, Nguyen TH. Effect of leaf surface chemical properties on the efficacy of sanitizer for rotavirus inactivation. Applied and Environmental Microbiology 2016;82(20):6214-6222. |
R835826 (2016) R835826 (2017) R835826 (2019) R835826 (Final) |
Exit Exit Exit |
|
|
Romero-Maraccini OC, Shisler JL, Nguyen TH. Solar and temperature treatments affect the ability of human rotavirus wa to bind to host cells and synthesize viral RNA. Applied and Environmental Microbiology 2015;81(12):4090-4097. |
R835826 (2016) R835826 (2019) R835826 (Final) |
Exit Exit Exit |
Supplemental Keywords:
Drinking water, pathogen, wastewater, disinfection system;Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.