Grantee Research Project Results

2017 Progress Report: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse

EPA Grant Number: R835826
Title: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse
Investigators: Nguyen, Thanh (Helen) H. , Shisler, Joanna L , Guest, Jeremy S
Institution: University of Illinois Urbana-Champaign
EPA Project Officer: Spatz, Kyle
Project Period: September 1, 2015 through August 31, 2018 (Extended to August 31, 2020)
Project Period Covered by this Report: September 1, 2016 through August 31,2017
Project Amount: $750,000
RFA: Human and Ecological Health Impacts Associated with Water Reuse and Conservation Practices (2014) RFA Text | Recipients Lists
Research Category: Human Health , Water

Objective:

1) Determine the molecular mechanisms responsible for virus inactivation; 2) Determine factors required for effective virus inactivation by natural sunlight and UVC; and 3) Develop pond and UVC design guidelines to achieve reliable virus inactivation and elucidate trade-offs across and within dimensions of sustainability.

Progress Summary:

Both rotavirus genetics and the formation of reactive oxygen species by wastewater organic matter will control rotavirus inactivation under solar irradiation
In the absence of wastewater exogenous sensitizers, solar irradiation caused damage to the VP4 protein.
In the presence of wastewater exogenous sensitizers that formed reactive oxygen species upon solar irradiation, mutagenesis of capsid proteins like VP2, VP6, and VP7 destabilize the capsid to allow permeabilization.
UV254 nm irradiation has lower inactivation efficacy compared to UV220 nm irradiation.
The presence of algal organic matter did not influence rotavirus inactivation by UV254 nm irradiation, but it increased RV inactivation by UV220 nm irradiation.
Inactivation efficacy by UV220 nm did not depend on rotavirus strains.

Future Activities:

Complete Task 3 to determine mechanisms of virus inactivation by UV220nm and UV254nm for wastewater.
Develop a framework to predict rotavirus inactivation based on sequencing of VP4, 2, 6, and 7.
Develop pond and UVC design guidelines to achieve reliable virus inactivation.
Communicate the findings with the stakeholders.

Journal Articles on this Report : 4 Displayed | Download in RIS Format

Publications Views
Other project views:	All 27 publications	11 publications in selected types	All 11 journal articles

Publications
Type	Citation	Project	Document Sources
Journal Article	Feng Z, Lu R, Yuan B, Zhou Z, Wu Q, Nguyen TH. Influence of solution chemistry on the inactivation of particle-associated viruses by UV irradiation. Colloids and Surfaces B: Biointerfaces 2016;148:622–628.	R835826 (2016) R835826 (2017) R835826 (2019) R835826 (Final)	Abstract from PubMed Full-text: ScienceDirect - Full Text HTML Exit Abstract: ScienceDirect-Abstract Exit Other: ResearchGate-Abstract Exit
Journal Article	Fuzawa M, Ku K-M, Palma-Salgado SP, Nagasaka K, Feng H, Juvik JA, Sano D, Shisler JL, Nguyen TH. Effect of leaf surface chemical properties on the efficacy of sanitizer for rotavirus inactivation. Applied and Environmental Microbiology 2016;82(20):6214-6222.	R835826 (2016) R835826 (2017) R835826 (2019) R835826 (Final)	Abstract from PubMed Full-text: ASFM - Full Text PDF Exit Abstract: AEM-Abstract Exit Other: ResearchGate-Abstract Exit
Journal Article	Lu, R., Zhang, C., Piatkovsky, M., Ulbricht, M., Herzberg, M., Nguyen, TH., Improvement of virus removal using ultrafiltration membranes modified with grafted zwitterionic polymer hydrogels, Water research 116, 86-94, doi:10.1016/j.watres.2017.03.023.	R835826 (2017)	Abstract from PubMed Full-text: Science Direct - Full Text HTML Exit Abstract: Abstract
Journal Article	Romero-Maraccini, OC, Shisler, JL, Nguyen., TH., Solar and Temperature Treatments Affect the Ability of Human Rotavirus Wa To Bind to Host Cells and Synthesize Viral RNA, Applied and environmental microbiology 2015;81(12):4090-7	R835826 (2017)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: ASFM - Full Text HTML Exit Abstract: ASFM - Abstract HTML Exit Other: ASFM - Full Text PDF Exit

Supplemental Keywords:

drinking water, pathogen.

Progress and Final Reports:

Original Abstract

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.