Grantee Research Project Results
2018 Progress Report: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse
EPA Grant Number: R835826Title: Enabling Adaptive UV and Solar-Based Disinfection Systems to Reduce the Persistence of Viral Pathogens in Wastewater for Sustainable Reuse
Investigators: Nguyen, Thanh (Helen) H. , Shisler, Joanna L , Guest, Jeremy S
Institution: University of Illinois Urbana-Champaign
EPA Project Officer: Spatz, Kyle
Project Period: September 1, 2015 through August 31, 2018 (Extended to August 31, 2020)
Project Period Covered by this Report: September 1, 2017 through August 31,2018
Project Amount: $750,000
RFA: Human and Ecological Health Impacts Associated with Water Reuse and Conservation Practices (2014) RFA Text | Recipients Lists
Research Category: Human Health , Water
Objective:
Objectives of Research:
(1) Determine the molecular mechanisms responsible for virus inactivation; (2) determine factors required for effective virus inactivation by natural sunlight and Ultraviolet C Light (UVC); and (3) develop pond and UVC design guidelines to achieve reliable virus inactivation and elucidate tradeoffs across and within dimensions of sustainability.
Progress Summary:
We found the following results:
- Inactivation efficacy by UV 220 nm was significantly higher than by UV 254 irradiation for rotavirus (RV) (5 and 2 log10 reduction for UV dose of 22.5 mJ/cm2) but comparable for Tulane virus (TV) (5 log10 reduction for UV dose of 30 mJ/cm2).
- While damage in both genome and the protein responsible for binding was observed for UV irradiated single layer TV, only genome damage was detected for triple layer RT.
- While damage in both genome and the protein responsible for binding was observed for UV irradiated single layer TV, only genome damage was detected for triple layer RT.
- The results present here suggest that 220 nm irradiation is more robust than 254 nm irradiation for viral disinfection. Thus, knowledge of virus genome and protein structure should be considered for the design of UV disinfection process to produce safe treated water.
- The uncertainties of four parameters (hour of the day, water depth, location, and non-purgeable organic carbon concentration of the water body) accounted for most of the variance in total inactivation rate constant.
Future Activities:
Future Activities:
1.Complete study on inactivation mechanisms of rotaviruses and Tulane viruses by UV irradiation in the presence of algal organic matter
2.Prepare peer-reviewed publication.
3.Develop pond and UVC design guidelines to achieve reliable virus inactivation.
4.Disseminate the results.
Journal Articles on this Report : 2 Displayed | Download in RIS Format
| Other project views: | All 27 publications | 11 publications in selected types | All 11 journal articles |
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Araud E, Shisler JL, Nguyen TH. Inactivation mechanisms of human and animal rotaviruses by solar UVA and visible light. Environmental Science & Technology 2018;52:5682-5690 |
R835826 (2018) R835826 (2019) R835826 (Final) |
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Wang Y, Araud E, Shisler JL, Nguyen TH, Yuan B. Influence of algal organic matter on MS2 bacteriophage inactivation by ultraviolet irradiation at 220 nm and 254 nm. Chemosphere 2019;214:195-202 |
R835826 (2018) R835826 (2019) R835826 (Final) |
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Supplemental Keywords:
Drinking water, pathogenProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.