Main Title |
Use of a Human/Mouse Hybrid Cell Line to Detect Aneuploidy Induced by Environmental Chemicals. |
Author |
Athwal, R. S. ;
Sandhu, S. S. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;New Jersey Medical School, Newark. Dept. of Microbiology. |
Year Published |
1985 |
Report Number |
EPA/600/J-85/322; |
Stock Number |
PB86-162369 |
Additional Subjects |
Toxicology ;
Mice ;
Humans ;
Detection ;
Reprints ;
Cell lines ;
Aneuploidy
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-162369 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
A short-term assay utilizing a human/mouse monochromosomal hybrid cell line R3-5, to detect chemically induced aneuploidy in mammalian cells is described. A single human chromosome transferred into mouse cells was used as a cytogenetic marker to quantitate abnormal chromosome segregation following chemical treatment. The human chromosome present in the mouse cells can be readily identified by differential staining procedures. The frequency of cells containing 0 or 2 human chromosomes in the progeny of chemically-treated monochromosomal hybrid cells provided a direct measure of aneuploidy. The authors tested the sensitivity of the proposed system with three model chemicals (Colcemid, cyclophosphamide and benomyl) known to induce numerical or structural changes in chromosomes. The frequency of an abnormal segregation of the human chromosome was found to be dose dependent and consistently higher than controls. This system has the capability to detect gain as well as loss of a chromosome resulting from nondisjunction or other mechanisms leading to aneuploidy. |
Supplementary Notes |
Pub. in Mutation Research 149, p73-81 Feb 85. Prepared in cooperation with New Jersey Medical School, Newark. Dept. of Microbiology. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Use of a Human/Mouse Hybrid Cell Line to Detect Aneuploidy Induced by Environmental Chemicals. |
PUB Date Free Form |
c1985 |
Category Codes |
6T; 6C; 57Y; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
611417751 |
Origin |
NTIS |
Type |
CAT |