Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 39 OF 58

OLS Field Name OLS Field Data
Main Title Phenol and Trichloroethylene Degradation by 'Pseudomonas cepacia' G4: Kinetics and Interactions between Substrates.
Author Folsom, B. R. ; Chapman, P. J. ; Pritchard, P. H. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA-R-68033479; EPA/600/J-90/110;
Stock Number PB90-264201
Additional Subjects Vinyl chloride ; Phenol ; Biodeterioration ; Kinetics ; Enzymes ; Reprints ; Pseudomonas cepacia ; Substrate specificity
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-264201 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 12/03/1990
Collation 9p
Abstract Intact cells of Pseudomonas cepacia strain G4 completely degraded trichloroethylene (TCE) following growth with phenol. Degradation kinetics were determined for both phenol, used to induce requisite enzymes, and TCE, the target substrate. At phenol concentrations above 50 microM, phenol degradation was inhibited, yielding an apparent K(sub i) of 0.45 mM as modeled by the Haldane expression. To eliminate experimental problems associated with TCE volatility and partitioning, a 'no-headspace' assay was developed allowing for direct and accurate determinations of aqueous TCE concentration. Using this assay procedure, apparent K(s) and V(max) values determined for TCE degradation by intact cells were 5.6 microM and 7.9 nmole/(min times mg protein) respectively. Following a transient lag period, P. cepacia G4 degraded TCE at concentrations of at least 300 microM TCE with no apparent retardation in rate.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v56 n5 p1279-1285 May 90. Also pub. as Environmental Research Lab., Gulf Breeze, FL. rept. no. CONTRIB-687. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Phenol and Trichloroethylene Degradation by 'Pseudomonas cepacia' G4: Kinetics and Interactions between Substrates.
Category Codes 57K; 57B
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 029924538
Origin NTIS
Type CAT