Main Title |
Quantitative Analysis of the Metabolism of 9,10-Dihydrobenzo(a)pyrene by Induced Rat Liver Microsomes. |
Author |
Nesnow, S. ;
Jackson, L. ;
Padgett, W. T. ;
Lambert, G. R. ;
Agarwal, S. C. ;
|
CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA-68-D1-0148; EPA/600/J-94/076; |
Stock Number |
PB94-141447 |
Additional Subjects |
Metabolism ;
Liver microsomes ;
Toxicity ;
Rats ;
Mutagens ;
Carcinogens ;
Benzo(a)pyrene ;
Kinetics ;
Reprints ;
Pyrene/dihydrobenzo(a)
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-141447 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
The ability of reduced polycyclic aromatic hydrocarbons to be converted to their fully aromatic forms by the microsomal cytochrome P-450 mixed-function oxidases may assist in the explanation of the mutagenic and tumorigenic activities of these agents. The metabolic conversion of 9,10-dihydrobenzo(a)pyrene (9,10-DHB(a)P) to benzo(a)pyrene (B(a)P) and 9- and/or 10-hydroxy-9,10-DHB(a)P (OH-9,10-DHB(a)P) was quantitatively measured. In beta-naphtho-flavone-induced rat liver microsomes, 9,10-DHB(a)P was metabolized to B(a)P with a specific activity of 1.51 nmol B(a)P formed/min/mg microsomal protein. The formation of B(a)P was directly related to incubation time and microsomal protein concentration. Similarly, 9,10-DHB(a)P was converted to OH-9,10-DHB(a)P with a specific activity of 4.48 nmol OH-9,10-DHB(a)P formed/min/mg microsomal protein. Its formation was directly related to incubation time and microsomal protein concentration. The possibility of OH-9,10-DHB(a)P as a metabolic intermediate to B(a)P is discussed. (Copyright (c) 1993 Elsevier Scientific Publishers Ireland Ltd.) |
Supplementary Notes |
Pub. in Cancer Letters, v73 n2-3 p135-140, 30 Sep 93. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Quantitative Analysis of the Metabolism of 9,10-Dihydrobenzo(a)pyrene by Induced Rat Liver Microsomes. |
Category Codes |
57Y; 57B |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
407722549 |
Origin |
NTIS |
Type |
CAT |