Main Title |
Effects of Physicochemical Factors and Bacterial Colony Morphotype on Association of 'Vibrio vulnificus' with Hemocytes of 'Crassostrea virginica'. |
Author |
Harris-Young, L. ;
Tamplin, M. L. ;
Fisher, W. S. ;
Mason, J. W. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Alabama Univ. in Birmingham. School of Public Health. ;Food and Drug Administration, Dauphin Island, AL. Fishery Research Branch. ;Florida Univ., Gainesville.;National Oceanic and Atmospheric Administration, Rockville, MD. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-94/012; |
Stock Number |
PB94-140688 |
Additional Subjects |
Bacteria ;
Diseases ;
Humans ;
Oysters ;
Food consumption ;
Tolerances(Physiology) ;
Temperature ;
Concentration(Composition) ;
Serum ;
Tissues(Biology) ;
Estuaries ;
Reprints ;
Crassostrea virginica ;
Hemocytes
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-140688 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
Vibrio vulnificus is a naturally occurring marine bacterium which causes invasive disease of immunocompromised humans following consumption of raw oysters. It is natural flora of Gulf Coast estuaries and has been found to inhabit tissues of oysters, Crassostrea virginica (Gmelin, 1791). The interaction of V. vulnificus with oyster host defenses has not been reported in detail. In the present studies, we examined the interaction of V. vulnificus with phagocytic oyster hemocytes in response to time, temperature, bacterial concentration, pretreatment in hemolymph, and with V. vulnificus translucent and opaque colonial morphotypes. Within these experimental parameters, results showed that association of V. vulnificus with hemocytes increased with time, temperature, and V. vulnificus:hemocyte ratio. Pretreatment of V. vulnificus with serum, or increased serum concentration, did not enhance V. vulnificus-hemocyte associations, indicating undetectable opsonic activity. More than 50% of hemocytes bound the translucent, avirulent morphotype, whereas 10% to 20% were associated with the opaque, virulent form. (Copyright (c) 1993, American Society for Microbiology.) |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v59 n4 p1012-1017 Apr 93. Prepared in cooperation with Alabama Univ. in Birmingham. School of Public Health, Food and Drug Administration, Dauphin Island, AL. Fishery Research Branch, and Florida Univ., Gainesville. Sponsored by National Oceanic and Atmospheric Administration, Rockville, MD. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Effects of Physicochemical Factors and Bacterial Colony Morphotype on Association of 'Vibrio vulnificus' with Hemocytes of 'Crassostrea virginica'. |
Category Codes |
57K; 98F; 57Y |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
406331287 |
Origin |
NTIS |
Type |
CAT |