| Main Title |
Method development and preliminary applications for leptospira spirochetes in water samples / |
| Author |
M. WALKER
|
| Other Authors |
|
| CORP Author |
Nevada Univ., Reno.; Environmental Protection Agency, Research Triangle Park, NC. National Homeland Security Research Center. |
| Publisher |
U.S. Environmental Protection Agency, |
| Year Published |
2008 |
| Report Number |
EPA/600/R-08/017 |
| Stock Number |
PB2008-108874 |
| Subjects |
Leptospira spirochetes ;
Decontamination (from gases, chemicals, etc) ;
Drinking water--Analysis
|
| Additional Subjects |
Leptospira ;
Water sampling ;
Infectious diseases ;
Zoontic diseases ;
Humans ;
Animals ;
Contamination ;
Detection ;
Antibodies ;
Occurrence ;
Recrational areas ;
Watersheds ;
Hawaiian Islands ;
Leptospira spirochetes
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB2008-108874 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
[30] : digital, PDF file |
| Abstract |
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to (1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, (2) develop a protocol to isolate Leptospira spirochetes from water samples, and (3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands. |
| Notes |
"EPA/600/R-08/017 "February 2008." Title taken from title screen (viewed Nov 5, 2008). |
| Contents Notes |
Leptospirosis is an increasingly important zoonosis that infects humans and animals through contact with contaminated water. The primary challenges for carrying out water sampling for Leptospira spirochetes include isolation, concentration, and quantitative detection of small numbers of target organisms in water. The objectives of this work were to 1) develop a protocol to apply fluorescently labeled antibodies to water samples to detect virulent serovars of Leptospira, 2) develop a protocol to isolate Leptospira spirochetes from water samples, and 3) use these techniques to assess the occurrence of Leptospira in recreational waters in watersheds on Oahu and Kauai, in the Hawaiian Islands. |
| Place Published |
Cincinnati, OH. |
| Supplementary Notes |
Sponsored by Environmental Protection Agency, Research Triangle Park, NC. National Homeland Security Research Center. |
| Availability Notes |
Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| Access Notes |
Mode of access: World Wide Web.; System requirements: Adobe Acrobat Reader. |
| Corporate Au Added Ent |
United States. Environmental Protection Agency. Office of Research and Development.; National Homeland Security Research Center (U.S.) |
| PUB Date Free Form |
Feb 2008 |
| Category Codes |
57E; 57K; 68D |
| NTIS Prices |
PC A03 |
| BIB Level |
m |
| Medium |
electronic resource |
| Cataloging Source |
OCLC/T |
| Control Number |
019000580 |
| OCLC Time Stamp |
20081105102453 |
| Language |
eng |
| Origin |
NTIS |
| Type |
MERGE |
| OCLC Rec Leader |
02200nam 2200397Ka 45020 |
