| Main Title |
Fluorometric Determination of the DNA (Deoxyribonucleic Acid) Concentration in Municipal Drinking Water. |
| Author |
McCoy, W. F. ;
Olson, B. H. ;
|
| CORP Author |
California Univ., Irvine.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. |
| Year Published |
1985 |
| Report Number |
EPA-R-809817; EPA/600/J-85/466; |
| Stock Number |
PB87-170460 |
| Additional Subjects |
Deoxyribonucleic acids ;
Bacteria ;
Water pollution ;
Fluorometers ;
Municipalities ;
Concentration(Composition) ;
Turbidity ;
Collecting methods ;
Chlorine ;
Assaying ;
Cells(Biology) ;
Reprints ;
Drinking water
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-170460 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
DNA concentrations in municipal drinking water samples were measured by fluorometry, using Hoechst 33258 fluorochrome. The concentration, extraction, and detection methods used were adapted from existing techniques. The method is reproducible, fast, accurate, and simple. The amounts of DNA per cell for five different bacterial isolates obtained from drinking water samples were determined by measuring DNA concentration and total cell concentration (acridine orange epifluorescence direct cell counting) in stationary pure cultures. The relationship between DNA concentration and epifluorescence total direct cell concentration in 11 different drinking water samples was linear and positive; the amounts of DNA per cell in these samples did not differ significantly from the amounts in pure culture isolates. (Copyright (c) 1985, American Society for Microbiology.) |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v49 n4 p811-817 Apr 85. Sponsored by Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Fluorometric Determination of the DNA (Deoxyribonucleic Acid) Concentration in Municipal Drinking Water. |
| PUB Date Free Form |
c1985 |
| Category Codes |
68D; 91A; 99A |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
716918627 |
| Origin |
NTIS |
| Type |
CAT |
