| Main Title |
Human Hepatocyte-Mediated Mutagenesis and DNA Repair Activity. |
| Author |
Strom, S. C. ;
Novicki, D. L. ;
Novotny, A. ;
Jirtle, R. L. ;
Michalopoulos, G. ;
|
| CORP Author |
Duke Univ. Medical Center, Durham, NC. |
| Year Published |
1983 |
| Report Number |
EPA-R-808549; EPA-600/J-83-216; |
| Stock Number |
PB84-206267 |
| Additional Subjects |
Deoxyribonucleic acid ;
Humans ;
Hepatocytes ;
Fibroblasts ;
Cells(Biology) ;
Metabolism ;
Reprints ;
Mutagenesis ;
Nitrosamine/diethyl
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-206267 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
Combined cultures of human hepatocytes and human fibroblasts constitute a system composed entirely of normal human cells that can be used to investigate the mutagenicity of chemicals requiring metabolic activation. Addition of diethylnitrosamine (DEN) to this system resulted in mutations at the hypoxanthine-guanine phosphoriboribosyltransferase locus of the human fibroblasts. In separate experiments with cultures of hepatocytes alone, DEN induced unscheduled DNA synthesis (UDS) in the human hepatocytes. A comparative analysis of UDS and hepatocyte-mediated mutagensis indicates a great degree of similarity between the human and previously studied rat hepatocytes in their response to DEN in vitro. |
| Availability Notes |
Pub. in Carcinogenesis, v4 n6 p683-686 1983. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Human Hepatocyte-Mediated Mutagenesis and DNA Repair Activity. |
| PUB Date Free Form |
c1983 |
| Category Codes |
6T; 6C; 57Y; 57F |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
424418749 |
| Origin |
NTIS |
| Type |
CAT |
