||Biochemical Studies of Promoters of Carcinogenesis in Rat Liver.
Kitchin, K. T. ;
Brown., J. L. ;
||Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Lethal dosage ;
Deoxyribonucleic acids ;
Reference standards ;
DNA damage ;
Ornithine decarboxylase ;
Cytochrome P-450 ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Adult female rats were orally dosed with 1/5 the LD50 of either an initiator of carcinogens (1,2-dibromo-3-chloropropane (DBCP)), promoters or putative promoters of carcinogenesis (hexachlorobenzene, alpha-hexachlorocyclohexane, kepone and toxaphene) or noncarcinogens (coumaphos, EDTA, caprolactam, 8-hydroxyquinoline, titanium(IV)oxide, sodium diethyldithiocarbamate (DEDTC), and sucrose) at 21 and 4 hours before sacrifice. The initiator and promoters selected in the study were all of the halogenated hydrocarbon class. DBCP caused a large degree of hepatic DNA damage and an increase in hepatic ODC activity. At doses ranging between 1/5 to 3/5 the LD50, all four promoters or putative promoters induced rat hepatic ODC activity. The seven noncarcinogens produced several biochemical effects at doses of 1/5 the LD50: serum alaine aminotransferase (SGPT) increases (caprolactam and DEDTC), decreased hepatic cytochrome P-450 (sucrose and DEDTC) and increased hepatic ODC (8-hydroxyquinoline and DEDTC). None of the seven noncarcinogens caused hepatic DNA damage or coordinate induction of hepatic ODC and cytochrome P-450.
||Pub. in Teratogenesis, Carcinogenesis, and Mutagenesis, v9 n5 p273-285 Oct 89.
|NTIS Title Notes
||Reprint: Biochemical Studies of Promoters of Carcinogenesis in Rat Liver.
||57Y; 57B; 68G
||PC A03/MF A01