Main Title |
Quantitative Evaluation of the Effects of Human Carcinogens and Related Chemicals of Human Foreskin Fibroblasts. |
Author |
Kurian, P. ;
Nesnow, S. ;
Milo., G. E. ;
|
CORP Author |
Ohio State Univ., Columbus. Dept. of Physiological Chemistry.;Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA-68-02-4141; EPA/600/J-90/137; |
Stock Number |
PB91-109751 |
Additional Subjects |
Carcinogens ;
Skin(Anatomy) ;
In vitro analysis ;
Liver ;
Fibroblasts ;
Reprints ;
Toxic substances ;
Metabolic activation ;
Cell survival ;
Dose-response relationships ;
Neoplastic cell transformation
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-109751 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
15p |
Abstract |
Twelve compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability of human skin fibroblasts in vitro in the presence and absence of human liver S-9 mix. In the absence of the human liver S-9 mix, only seven of the twelve compounds were highly cytotoxic in the 0-100 microgram/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboethoxyamino)-1,4-benzoquinone (AZQ) > cis-platin > N, N-bis(2-chloroethyl)-2-naphthylamine > bis(chloromethyl)-ether (BCME) > acrylonitrile > styrene oxide > aflatoxin B1 (AFB1). The other five compounds, aflatoxin B2 (AFB2), methylmethacrylate, 1-naphthalamine, 2-naphthylamine, and cyclophosphamide, exhibited less than 40% inhibition of colony formation even at 100 microgram/ml of the compound (the maximum concentration of AFB2 used was 50 microgram/ml due to its poor solubility). Cytotoxicity was not significantly altered in the presence of human liver S-9 mix except for AFB1 and styrene oxide treatment. There was a drastic increase in cytotoxicity following treatment with 1 microgram/ml or more AFB1 and a significant reduction in cytotoxicity following treatment with 100 microgram/ml styrene oxide in the presence of S-9 mix. Anchorage independent growth (AI) of treated cells in soft agar was used as a biological end point for the expression of chemical transformation. (Copyright (c) 1990 Princeton Scientific Publishing Co., Inc.) |
Supplementary Notes |
Pub. in Jnl. of Cell Biology and Toxicology, v6 n2 p171-184 May 90. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Quantitative Evaluation of the Effects of Human Carcinogens and Related Chemicals of Human Foreskin Fibroblasts. |
Category Codes |
57Y; 68G |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
036519357 |
Origin |
NTIS |
Type |
CAT |