| Main Title |
Mutation Spectra of Glu-P-1 in Salmonella: Induction of Hotspot Frameshifts and Site-Specific Base Substitutions. |
| Author |
Levine, J. G. ;
Knasmueller, S. ;
Shelton, M. L. ;
DeMarini, D. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. ;Vienna Univ. (Austria). Inst. fuer Tumorbiologie und Krebsforschung. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/552; |
| Stock Number |
PB95-148136 |
| Additional Subjects |
Frameshift mutation ;
Site-directed mutagenesis ;
Salmonella typhimurium ;
Mutagens ;
DNA adducts ;
Sequence deletion ;
Carcinogens ;
Alleles ;
Dose-response relationships ;
Reprints ;
Imidazole/2-amino-6-methyldipyrido(1-2-a-3'-2'-d)
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-148136 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
14p |
| Abstract |
The authors used colony probe hybridization and PCR/DNA sequence analysis to determine the mutations in approximately 1,640 revertants of the -1 frameshift allele hisD3052 and approximately 260 revertants of the base substitution allele hisG46 of Salmonella typhimurium induced by the heterocyclic amine cooked food mutagen 2-amino-6-methyldipyrido(1,2-a:3',2'-d)imidazole (Glu-P-1). All of the mutations were at sites containing guanine, which is the base at which Glu-P-1 forms DNA adducts. A hotspot mutation involving the deletion of a CG or GC within the sequence CGCGCGCG accounted for 100% of the Glu-P-1-induced mutations at the frameshift allele in strains TA1978 (uvr(+)) and TA1538 (delta uvrB) and 99% in TA98 (delta uvrB, pKM101). To explain the induction of these hotspot mutations by Glu-P-1, the authors describe here a more detailed version of their recently proposed correct incorporation/slippage model. (Copyright (c) 1994 Wiley-Liss, Inc.) |
| Supplementary Notes |
Pub. in Environmental and Molecular Mutagenesis 24, n1 p11-22 Aug 94. Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. and Vienna Univ. (Austria). Inst. fuer Tumorbiologie und Krebsforschung. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Mutation Spectra of Glu-P-1 in Salmonella: Induction of Hotspot Frameshifts and Site-Specific Base Substitutions. |
| Category Codes |
57Y; 57F; 57K |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/00 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
503414753 |
| Origin |
NTIS |
| Type |
CAT |
