| Main Title |
Morphological Transformation and DNA Adduct Formation by Benz(j)aceanthrylene and Its Metabolites in C3H10T1/2CL8 Cells: Evidence for Both Cyclopenta-Ring and Bay-Region Metabolic Activation Pathways. |
| Author |
Nesnow, S. ;
Lasley, J. ;
Curti, S. ;
Ross, J. ;
Nelson, G. ;
|
| CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA-68-02 4456; EPA/600/J-92/062; |
| Stock Number |
PB92-150762 |
| Additional Subjects |
Mutagens ;
DNA adducts ;
Metabolism ;
Aromatic polycyclic hydrocarbons ;
Cultured cells ;
Metabolic activation ;
Phosphorus 32 ;
Cell survival ;
Cell morphology ;
Thin layer chromatography ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-150762 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
Benz(j)aceanthrylene (B(j)A), a cyclopenta-fused polycyclic aromatic hydrocarbon related to 3-methylcholanthrene, has been studied to identify the major routes of metabolic activation in transformable C3H10T1/2CL8(C3H10T1/2) mouse embryo fibroblasts in culture. Previous studies have reported that the major (55% of total) B(j)A metabolite formed by C3H10T1/2 cells was (+ or -)-trans-9,10-dihydroxy-B(j)A(B(jA-9,10-diol), the dihydrodiol in the bay-region ring, with moderate amounts (14% of total) of (+ or -)-trans-1,2-dihydro-1,2-dihydroxy-B(j)A (B(j)A-1,2-diol), the cyclopenta-ring dihydrodiol. Qualitative and quantitative analysis of the postlabeling data suggests that B(j)A is metabolically activated by two distinct routes, the bay-region diol-epoxide route and the cyclopenta-ring oxide route, the former being the most significant. |
| Supplementary Notes |
Pub. in Jnl. of Cancer Research, v51 p6163-6169, 15 Nov 91. See also PB85-124709, PB89-109797 and PB90-103458. Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. Sponsored by Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Morphological Transformation and DNA Adduct Formation by Benz(j)aceanthrylene and Its Metabolites in C3H10T1/2CL8 Cells: Evidence for Both Cyclopenta-Ring and Bay-Region Metabolic Activation Pathways. |
| Category Codes |
57Y; 57F; 57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
211522896 |
| Origin |
NTIS |
| Type |
CAT |
