| Main Title |
Phenol and Trichloroethylene Degradation by 'Pseudomonas cepacia' G4: Kinetics and Interactions between Substrates. |
| Author |
Folsom, B. R. ;
Chapman, P. J. ;
Pritchard, P. H. ;
|
| CORP Author |
Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA-R-68033479; EPA/600/J-90/110; |
| Stock Number |
PB90-264201 |
| Additional Subjects |
Vinyl chloride ;
Phenol ;
Biodeterioration ;
Kinetics ;
Enzymes ;
Reprints ;
Pseudomonas cepacia ;
Substrate specificity
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-264201 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Intact cells of Pseudomonas cepacia strain G4 completely degraded trichloroethylene (TCE) following growth with phenol. Degradation kinetics were determined for both phenol, used to induce requisite enzymes, and TCE, the target substrate. At phenol concentrations above 50 microM, phenol degradation was inhibited, yielding an apparent K(sub i) of 0.45 mM as modeled by the Haldane expression. To eliminate experimental problems associated with TCE volatility and partitioning, a 'no-headspace' assay was developed allowing for direct and accurate determinations of aqueous TCE concentration. Using this assay procedure, apparent K(s) and V(max) values determined for TCE degradation by intact cells were 5.6 microM and 7.9 nmole/(min times mg protein) respectively. Following a transient lag period, P. cepacia G4 degraded TCE at concentrations of at least 300 microM TCE with no apparent retardation in rate. |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v56 n5 p1279-1285 May 90. Also pub. as Environmental Research Lab., Gulf Breeze, FL. rept. no. CONTRIB-687. Sponsored by Environmental Research Lab., Gulf Breeze, FL. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Phenol and Trichloroethylene Degradation by 'Pseudomonas cepacia' G4: Kinetics and Interactions between Substrates. |
| Category Codes |
57K; 57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
029924538 |
| Origin |
NTIS |
| Type |
CAT |
