||Cigarette Smoke-Induced DNA Adducts in the Respiratory and Nonrespiratory Tissues of Rats.
Gairola, C. G. ;
Gupta, R. C. ;
||Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
DNA adducts ;
Aryl hydrocarbon hydroxylases ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Formation of DNA adducts is regarded as an essential initial step in the process of chemical carcinogenesis. To determine how chronic exposure to cigarette smoke affects the distribution of DNA adducts in selected respiratory and nonrespiratory tissues, the authors exposed male Sprague-Dawley rats daily to fresh mainstream smoke from the University of Kentucky reference cigarettes (2Rl) in a nose-only exposure system for 32 weeks. Blood carboxyhemoglobin, total particulate matter (TPM) intake, and pulmonary aryl hydrocarbon hydroxylase values indicated effective exposure of animals to cigarette smoke. DNA was extracted from three respiratory (larynx, trachea, and lung) and three nonrespiratory (liver, heart, and bladder) tissues and analyzed for DNA adducts by (32)P-postlabeling assay under conditions capable of detecting low levels of diverse aromatic/hydrophobic adducts. Data showed that the total DNA adducts in the lung, heart, trachea, and larynx were increased by 10- to 20-fold in the smoke-exposed group. Five-fold increase was observed in the bladder tissue, but differences were not present in the liver DNA of control and smoke-exposed groups. (Copyright (c) 1991 Wiley-Liss, Inc.)
||Pub. in Environmental and Molecular Mutagenesis, v17 p253-257, 1991. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Cigarette Smoke-Induced DNA Adducts in the Respiratory and Nonrespiratory Tissues of Rats.
||PC A02/MF A01