||Carcinogen-DNA Adducts in Cultures of Rat and Human Hepatocytes.
Monteith, D. K. ;
Gupta, R. C. ;
||Indiana Univ.-Purdue Univ. at Fort Wayne. Dept. of Biological Sciences.;Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
DNA adducts ;
Aminobiphenyl compounds ;
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||Exposure to chemical carcinogens can often be identified by detection of DNA adduct lesions. Primary cultures of isolated rat and human hepatocytes were exposed to 2-acetyl-aminofluorene (AAF), 4-aminobiphenyl (ABP), or benzo(a)pyrene (BP). The isolated DNA (32)P-postlabeling assay. A greater total of carcinogen-DNA adducts, 2-12-fold, were observed in human hepatocytes than rate hepatocytes at the same concentrations. The predominant DNA adducts for each carcinogen were the same between rat and human cells. The N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) was the major AAF-DNA adduct. The N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG-C8-ABP) was the major ABP-DNA adduct. In the rat N2-(10 beta, 8 alpha, 9 alpha-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yl)deoxyguanosine (dG-N2-BP) and two unidentified adducts were nearly equivalent in amount, while the major BP-DNA adducts in the humans was the dG-N2-BP. The rat hepatocyte in vitro results are comparable to the predominant adducts found with rats exposed in vivo. The two different cultures of human hepatocytes demonstrated qualitative and quantitative differences in specific DNA adducts from rat hepatocytes.
||Pub. in Jnl. of Cancer Letters, v62 p87-93 1992. Sponsored by Kentucky Univ., Lexington. Graduate Center for Toxicology, and Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Carcinogen-DNA Adducts in Cultures of Rat and Human Hepatocytes.
||PC A02/MF A01