| Main Title |
Carcinogen-DNA Adducts in Cultures of Rat and Human Hepatocytes. |
| Author |
Monteith, D. K. ;
Gupta, R. C. ;
|
| CORP Author |
Indiana Univ.-Purdue Univ. at Fort Wayne. Dept. of Biological Sciences.;Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-92/201; |
| Stock Number |
PB92-189075 |
| Additional Subjects |
Carcinogens ;
DNA adducts ;
Liver ;
Rats ;
Humans ;
Cells(Biology) ;
2-acetylaminofluorene ;
Aminobiphenyl compounds ;
Benzo(a)pyrene ;
Autoradiography ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-189075 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Exposure to chemical carcinogens can often be identified by detection of DNA adduct lesions. Primary cultures of isolated rat and human hepatocytes were exposed to 2-acetyl-aminofluorene (AAF), 4-aminobiphenyl (ABP), or benzo(a)pyrene (BP). The isolated DNA (32)P-postlabeling assay. A greater total of carcinogen-DNA adducts, 2-12-fold, were observed in human hepatocytes than rate hepatocytes at the same concentrations. The predominant DNA adducts for each carcinogen were the same between rat and human cells. The N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) was the major AAF-DNA adduct. The N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG-C8-ABP) was the major ABP-DNA adduct. In the rat N2-(10 beta, 8 alpha, 9 alpha-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yl)deoxyguanosine (dG-N2-BP) and two unidentified adducts were nearly equivalent in amount, while the major BP-DNA adducts in the humans was the dG-N2-BP. The rat hepatocyte in vitro results are comparable to the predominant adducts found with rats exposed in vivo. The two different cultures of human hepatocytes demonstrated qualitative and quantitative differences in specific DNA adducts from rat hepatocytes. |
| Supplementary Notes |
Pub. in Jnl. of Cancer Letters, v62 p87-93 1992. Sponsored by Kentucky Univ., Lexington. Graduate Center for Toxicology, and Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Carcinogen-DNA Adducts in Cultures of Rat and Human Hepatocytes. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
220521629 |
| Origin |
NTIS |
| Type |
CAT |
