||Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.
Kurelec, B. ;
Krca, S. ;
Garg, A. ;
Gupta, R. C. ;
||Institut Rudjer Boskovic, Zagreb (Yugoslavia). Center for Marine Research. ;Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Metabolic activation ;
Deoxyribonucleic acids ;
Mixed function oxidases ;
Liquid chromatography ;
Phosphorus 32 ;
Aromatic polycylic hydrocarbons ;
Toxic substances ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||The authors have investigated the formation of DNA adducts in starved, fed, and 5,6-benzoflavone-pretreated carp following i.p. administration of benzo(a)pyrene. (32)P-postlabeling analysis of the liver DNAs showed the presence of one predominant (>92%) adduct in all three groups. Cochromatography experiments revealed that the main adduct was identical to authentic BPDEI-dG (10(beta)-(deoxyguanosin-N2-yl)-7(beta), 8(alpha), 9(alpha)-trihydroxy-7,8,0,10-tetrahydrobenzo(a)pyrene). The formation of the adduct was evident as early as 1.5 h post-treatment and the levels increased steadily up to 7 days, reaching about 125, 110, and 102 attomole/microgram DNA in starved, fed, and benzoflavone-pretreated carp, respectively. During this period, the benzo(a)pyrene-induced benzo(a)pyrene monooxygenase activity increased from the uninduced, natural level of about 3 pmol/mg per min to levels of 35, 62, and 79 pmol/mg per min in starving, fed, and 5,6-benzoflavone pretreated fish, respectively. A slow but steady formation of the diolepoxide-dG adduct was also observed in the liver DNA of carp following p.o. treatment. (Copyright (c) 1991 Elsevier Scientific Publishers Ireland Ltd.)
||Pub. in Jnl. Cancer Letters, v57 p255-260 1991. Prepared in cooperation with Kentucky Univ., Lexington. Graduate Center for Toxicology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.
||57B; 57F; 57Y
||PC A02/MF A01