||A fluorescence immunoassay technique for detecting organic environimental contaminants /
Lukens, Herbert R. ;
Williams, Colin B. ;
Levison, Stuart A. ;
Dandliker, Walter B. ;
||IRT Corp., San Diego, Calif.;National Environmental Research Center, Research Triangle Park, N.C.;Scripps Clinic and Research Foundation, La Jolla, Calif.
|| U.S. Environmental Protection Agency, Pesticides and Toxic Substances Effects Laboratory,
||EPA/650-1-75-004; INTEL-RT-8098-004; EPA-68-02-1266
Fluorescent antigen technique. ;
Fluorescent antibody technique.
Air pollution ;
Water pollution ;
Organic compounds ;
Antigen antibody reactions ;
Chemical compounds ;
Pollutant identification ;
Pesticide residues ;
||Region 3 Library/Philadelphia, PA
||Region 4 Library/Atlanta,GA
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||xii, 63 pages ; 28 cm.
The report describes the development and successful demonstration of an immunological assay for the detection of low molecular weight organic contaminants of environmental cocern. The specific technique described is a fluorescence polarization immunoassay, the theory of which is presented. The preparation of the two required reagents, namely a fluorescent conjugate of the contaminant of interest, together with an antibody to the contaminant, is described in detail. The specific contaminant chosen for this study was 2-aminobenzimidazole (MW = 133), a metabolite of certain fungicide agents used in agriculture. The particular fluorescent moiety chosen to form the conjugate with 2-aminobenzimidazole with fluorescein. A successful demonstration of the assay has been accomplished, and a detection sensitivity in the sub-nanogram/ml range obtained.
"EPA/650-1-75-004." "May 1975." EPA contract no. 68-02-1266, ROAP No. 21AFM, program element no. 1E1078; EPA project officer: Dr. Ronald L. Baron. Includes bibliographical references (page 35).