This document (Field Operations Manual) contains a brief introduction and procedures to follow at the base location and on-site, including methods for sampling water chemistry (grabs and in situ measurements), benthic macroinvertebrates, sediment composition and toxicity, fish tissue, a pathogen indicator, and physical habitat. These methods are based on the guidelines developed and followed in the Coastal 2000 and National Coastal Assessment Monitoring and Assessment Program (USEPA, 2001), Methods described in this document are to be used specifically in work relating to the National Coastal Condition Assessment. All Project Cooperators must follow these guidelines. Mention of trade names or commercial products in this document does not constitute endorsement or recommendation for use. Details on specific methods for site evaluation and sample processing can be found in the appropriate companion document.