||Methods for Measuring Mutagenicity in Urine of Rats Dosed with (14C)di(2-ethylhexyl) Phthalate with Cover Letter.
||Eastman Kodak Co., Rochester, NY.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
||3 Feb 1984
Mutagenic pathways ;
In vitro analysis ;
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Di (2-ethylbexyl) phthalate (DEHP) is extensively used as a plasticizer for vinyl plastic articles. It has been found to be positive in an NCI rodent bioassay but has generally given negative results in vitro genotoxicity tests. We therefore decided to test the urine of rats fed (14 C) DEHP for mutagenic activity in the Ames Salmonella test. The recovery of radioactivity from the urine of rats dosed with (14 C) DEHP was examined by solvent extraction and XAD-2 resin absorption procedures. Both of these procedures were inadequate for quantitative recovery of urinary metabolites required for subsequent mutagenicity testing using the Ames Salmonella/microsome procedure. Recoveries of less than five percent were observed using standard solvent extraction techniques whereas the XAD-2 adsorption technique gave about 67 percent at high resin/urine ratios.