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RECORD NUMBER: 132 OF 395

OLS Field Name OLS Field Data
Main Title Estimation of Mutagenic/Carcinogenic Potential of Environmental Contaminants by Ion-Molecule Reactions and Tandem Mass Spectrometry.
Author Freeman, J. A. ; Johnson, J. V. ; Hail, M. E. ; Yost, R. A. ; Kuehl, D. W. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;Florida Univ., Gainesville. Dept. of Chemistry.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/028;
Stock Number PB90-217704
Additional Subjects Mass spectroscopy ; Deoxyribonucleic acids ; Tables(Data) ; Reprints ; Mutagenicity tests ; Carcinogenicity tests ; Environmental pollutants ; DNA damage
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-217704 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/27/1990
Collation 8p
Abstract
The ability to produce and detect products of model DNA/carcinogen ion-molecule reactions is demonstrated in the ion source and the collision cell of a triple quadrupole tandem mass spectrometer. Reaction between adenine and benzoyl chloride in the ion source is shown to produce the DNA adduct benzoyl adenine. The daughter ion mass spectrum of the reaction product is compared to that of the synthesized standard. Mass chromatograms of the reaction between mass-selected pyridine ions and various analytes eluting from a GC column into the collision cell are demonstrated and illustrate the ability to detect only the GC eluates that react with pyridine. This technique could provide a rapid and sensitive method for screening complex environmental samples for carcinogens, as well as for estimating the relative mutagenic/carcinogenic potential of environmental contaminants.