Record Display for the EPA National Library Catalog
RECORD NUMBER: 27 OF 52Main Title | Method for Determination of Low Levels of Exposure to 2,4-D and 2,4,5-T. | |||||||||||
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Author | Shafik, M. T. ; Sullivan, Hazel C. ; Enos., H. F. ; | |||||||||||
CORP Author | Environmental Protection Agency, Perrine, Fla. Perrine Primate Lab. | |||||||||||
Year Published | 1971 | |||||||||||
Stock Number | PB-276 306 | |||||||||||
Additional Subjects | Pesticides ; Chemical analysis ; Trace elements ; Herbicides ; Chlorine organic compounds ; Urine ; Laboratory animals ; Humans ; Rats ; Hydrolysis ; Electron capture ; Gas chromatography ; Reprints ; D2-4 herbicide ; T2-4-5 herbicide ; Acetic acid/dichlorophenoxy ; Acetic acid/trichlorophenoxy ; TCP 2-4-5 herbicide ; DCP 2-4 herbicide ; Phenol/trichloro ; Phenol/dichloro ; Urinalysis ; Electron capture gas chromatography ; Toxic substances | |||||||||||
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Collation | 11p | |||||||||||
Abstract | A method has been developed for the determination of trace quantities of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2,4-dichlorophenol (2,4-DCP), and 2,4,5-trichlorophenol (2,4,5-TCP) in human and rat urine. The method involves acid hydrolysis of the phenolic conjugates, extraction of the free phenols and acids, ethylation with diazoethane, silica-gel column chromatography clean-up of the derivatized urine extract, and gas chromatographic determination using the electron-capture detector. |