Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Enzyme-Immobilization Procedure for the Analysis of Enzyme-Inhibiting Chemicals in Water.
Author Christensen, G. M. ; Riedel, B. L. ;
CORP Author Environmental Research Lab.-Duluth, MN.
Year Published 1980
Report Number EPA-600/J-80-243;
Stock Number PB81-174427
Additional Subjects Urease ; Cholinesterase ; Water analysis ; Enzymes ; Sensitivity ; Reprints ; Water pollution detection ; Immobilized enzymes ; Enzyme technology
Library Call Number Additional Info Location Last
NTIS  PB81-174427 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 6p
The enzymes cholinesterase and urease were mixed individually with gelatin and immobilized onto the inside surface of glass capillary tubes. After the gelatin-enzyme mixture had dried, water samples containing various enzyme inhibiting test chemicals were pumped through the tubes to enable interaction to proceed between the foreign agents and the enzymes. Substrate for each enzyme was later passed through the tubes and any difference in relative enzyme activity determined. This procedure may be useful for health and environmental studies, particularly in the rapid testing for micro- and nanomolar quantities of enzyme-inhibiting pollutants in natural waters and complex effluents.