Main Title |
Identification of mutagenic components in wastewater effluents and sludges / |
Author |
Tabor, M. Wilson. ;
Loper, J. C.
|
Other Authors |
|
CORP Author |
Cincinnati Univ. Medical Center, OH.;Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory, |
Year Published |
1989 |
Report Number |
EPA/600/1-89/007; EPA-R-810792 |
Stock Number |
PB90-145731 |
Additional Subjects |
Sewage treatment ;
Waste water ;
Municipalities ;
Mutagens ;
Identifying ;
Salmonella ;
Bioassay ;
Assessments ;
Sludge disposal ;
Residues ;
Drinking water
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-145731 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
254 pages ; 28 cm |
Abstract |
Both industrially-impacted and domestic municipal sewage treatment plant wastewaters and sludges have been studied to isolate the residue organics for the characterization of their mutagenic properties and for the isolation/identification of the mutagenic components. Methods were investigated to isolate the residue organics for mutagenic assessment via the Salmonella/microsomal activation, i.e., 59, assay. Residue organics were isolated from wastewaters by a modification of the USEPA/EMSL-LV interim protocol for the isolation of residue organics from drinking water for mutagenic assessment. Residue organics were isolated from sludges via three methods: the Soxhlet extraction method of Hites; the USEPA/EMSL-CIN Method 624S/6255; and a newly-developed method in the study that features the milling of the sludge with anhydrous sodium sulfate to a homogeneous powder followed by sequential extractions using a solvent series from non-polar to polar. The residue organics from wastewaters and sludges were fractionated by high performance liquid chromatography (HPLC) using a coupled bioassay/analytical fractionation approach. In general, mutagenic assessment of the residue organics from the industrially-impacted treatment plant wastewaters and sludges show both direct acting and 59 dependent TA98 mutagenic activity. |
Notes |
Caption title. "November 1989." "EPA/600/1-89/007." "M. Kate Smith, project officer." "CR-810792." Microfiche. |