Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Human Hepatocyte-Mediated Mutagenesis and DNA Repair Activity.
Author Strom, S. C. ; Novicki, D. L. ; Novotny, A. ; Jirtle, R. L. ; Michalopoulos, G. ;
CORP Author Duke Univ. Medical Center, Durham, NC.
Year Published 1983
Report Number EPA-R-808549; EPA-600/J-83-216;
Stock Number PB84-206267
Additional Subjects Deoxyribonucleic acid ; Humans ; Hepatocytes ; Fibroblasts ; Cells(Biology) ; Metabolism ; Reprints ; Mutagenesis ; Nitrosamine/diethyl
Library Call Number Additional Info Location Last
NTIS  PB84-206267 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 6p
Combined cultures of human hepatocytes and human fibroblasts constitute a system composed entirely of normal human cells that can be used to investigate the mutagenicity of chemicals requiring metabolic activation. Addition of diethylnitrosamine (DEN) to this system resulted in mutations at the hypoxanthine-guanine phosphoriboribosyltransferase locus of the human fibroblasts. In separate experiments with cultures of hepatocytes alone, DEN induced unscheduled DNA synthesis (UDS) in the human hepatocytes. A comparative analysis of UDS and hepatocyte-mediated mutagensis indicates a great degree of similarity between the human and previously studied rat hepatocytes in their response to DEN in vitro.