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Main Title Quantitative Analysis of the Metabolism of 9,10-Dihydrobenzo(a)pyrene by Induced Rat Liver Microsomes.
Author Nesnow, S. ; Jackson, L. ; Padgett, W. T. ; Lambert, G. R. ; Agarwal, S. C. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Publisher c1993
Year Published 1993
Report Number EPA-68-D1-0148; EPA/600/J-94/076;
Stock Number PB94-141447
Additional Subjects Metabolism ; Liver microsomes ; Toxicity ; Rats ; Mutagens ; Carcinogens ; Benzo(a)pyrene ; Kinetics ; Reprints ; Pyrene/dihydrobenzo(a)
Library Call Number Additional Info Location Last
NTIS  PB94-141447 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The ability of reduced polycyclic aromatic hydrocarbons to be converted to their fully aromatic forms by the microsomal cytochrome P-450 mixed-function oxidases may assist in the explanation of the mutagenic and tumorigenic activities of these agents. The metabolic conversion of 9,10-dihydrobenzo(a)pyrene (9,10-DHB(a)P) to benzo(a)pyrene (B(a)P) and 9- and/or 10-hydroxy-9,10-DHB(a)P (OH-9,10-DHB(a)P) was quantitatively measured. In beta-naphtho-flavone-induced rat liver microsomes, 9,10-DHB(a)P was metabolized to B(a)P with a specific activity of 1.51 nmol B(a)P formed/min/mg microsomal protein. The formation of B(a)P was directly related to incubation time and microsomal protein concentration. Similarly, 9,10-DHB(a)P was converted to OH-9,10-DHB(a)P with a specific activity of 4.48 nmol OH-9,10-DHB(a)P formed/min/mg microsomal protein. Its formation was directly related to incubation time and microsomal protein concentration. The possibility of OH-9,10-DHB(a)P as a metabolic intermediate to B(a)P is discussed. (Copyright (c) 1993 Elsevier Scientific Publishers Ireland Ltd.)